重组大肠杆菌(毒素），NR-1_Vaccinia virus

NR-860?? Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli(Toxins)|Escherichia coli|Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli, NR-860."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-860 is a recombinant form of the B subunit of Shiga toxin type 1 (Stx1). The amino acid sequence includes a C-terminal hexa-histidine tag and amino acid residues 21 to 89 of the Stx1 subunit B protein (GenPept: AAA98348). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-860 has a theoretical molecular weight of approximately 8,513 daltons.Each vial of NR-860 contains approximately 1 mg of recombinant Stx1 subunit B suspended in phosphate buffered saline (pH 7.4). NR-860 lot 57680170 contains approximately 0.1 mg per vial suspended in phosphate buffered saline (pH 7.4).NR-860 reacts with mouse monoclonal antibody to Shiga toxin type 1 subunit B (BEI Resources NR-844).

NR-859?? Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli(Toxins)|Escherichia coli|Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli, NR-859."| Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-859 is a recombinant protein of the Shiga toxin type 1 subunit A (rStx1A). The protein sequence of the native Shiga toxin A subunit was modified to include an internal penta-histidine-tag used for the purpose of purification. The tagged protein was produced using a Qiagen QE30 series his-tag vector series, expressed in E. coli, and purified by nickel affinity chromatography. The resulting protein, NR-859, has a molecular weight of approximately 34,900 daltons. The predicted protein sequence of NR-859 is shown in Table 1 on the product sheet. Each vial contains 0.1 mg of NR-859 suspended in phosphate buffered saline. NR-859 reacts with rabbit polyclonal antiserum specific for Stx type 1 subunit A. Carbonic anhydrase negative control is not recognized.