产品描述信息
NR-686??SARS coronavirus, Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus (Proteins)|SARS coronavirus|Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus |4°C|NIH Biodefense and Emerging Infections Research Resources RepositoryAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus, NR-686."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
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NR-686 is a full-length, glycosylated recombinant form of the SARS-CoV spike (S) external envelope glycoprotein and contains a C-terminal histidine tag. A baculovirus signal sequence, MPLYKLLNVLWLVAVSNA, was added to the N-terminal end of the protein to enhance expression. The SARS-CoV spike (S) protein was produced in Sf9 insect cells using a baculovirus expression vector system and was purified using nickel affinity chromatography. The baculovirus signal sequence is efficiently removed during translocation of the protein across the membrane. The resulting protein, NR-686, has a molecular weight of approximately 160,000 daltons. The purified protein was demonstrated to be functionally active based on its ability to bind to a recombinant form of its functional receptor, angiotensin I converting enzyme-2.Each vial contains approximately 0.5 mg of NR-686 in 20 mM Tris-HCl (pH 8.0), 150 mM NaCl and 0.005% Tween-20.