NR-44080来自流感病毒的N9神经氨酸酶(NA)蛋白,A/Shanghai/1/2013(H7N9),来自杆状病毒的重组体(蛋白质）
产品图片
货号/SKU
NR-44080
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-44080?? N9 Neuraminidase (NA) Protein from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus(Proteins)|Influenza A virus|N9 Neuraminidase (NA) Protein from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus|2°C to 8°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N9 Neuraminidase (NA) Protein from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus, NR-44080."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the N9 neuraminidase (NA) protein from influenza A virus, A/Shanghai/1/2013 (H7N9) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 465 residues (GISAID EpiFlu: EPI439487).
Each vial contains 5 ?g to 50 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0), 500 mM NaCl, and 0.02% sodium azide.
NR-44080 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MUNANA).
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the N9 neuraminidase (NA) protein from influenza A virus, A/Shanghai/1/2013 (H7N9) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 465 residues (GISAID EpiFlu: EPI439487).
Each vial contains 5 ?g to 50 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0), 500 mM NaCl, and 0.02% sodium azide.
NR-44080 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
使用杆状病毒表达载体系统在SF9昆虫细胞中产生来自流感病毒，A /Shanga期/ 1/2013（H7N9）的N9神经氨酸酶（Na）蛋白的重组形式.将Na基因的预测的外胚瘤编码区域融合到编码N-末端六个组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶切割位点为四聚化结构域.通过镍亲和色谱法纯化蛋白质，然后用??凝血酶处理以除去四聚化结构域和组氨酸标签.在最终制剂之前进一步纯化凝血酶处理的蛋白质.全长Na前体蛋白是465个残基（GisaID EPIFlu：EPI439487）.
每个小瓶含有5μg至50μg纯化的重组Na蛋白，在50mM Tris（pH8.0），500mM NaCl和0.02％叠氮化钠中.
证明NR-44080基于其切割荧光底物2' - （4-甲基晶烯烃）-A-D-N-乙酰尿酰胺酸（4-MUNANA）的能力是在功能上活性的.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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