NR-15780结核分枝杆菌、CDC1551、转座子突变体敲除池8(突变细菌)
产品图片
货号/SKU
NR-15780
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15780??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 8(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 8|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 8, NR-15780."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 8 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 8 are available individually (see Pool 8 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 8 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 8 are available individually (see Pool 8 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
PROBABLE LIPOPROTEIN | MT1281 | Rv1244 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT0644 | Rv0614 | NA |
PROBABLE IRON-REGULATED SHORT-CHAIN DEHYDROGENASE/REDUCTASE | MT3321 | Rv3224 | NR-17572 |
CONSERVED HYPOTHETICAL PROTEIN | MT3461 | Rv3353c | NA |
PROBABLE OXIDOREDUCTASE | MT1767 | Rv1726 | NA |
POSSIBLE CONSERVED TRANSMEMBRANE PROTEIN | MT3561 | Rv3453 | NR-17586 |
PROBABLE TWO COMPONENT SENSOR HISTIDINE KINASE | MT3218 | Rv3132c | NR-15739 |
PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN | MT2795 | Rv2723 | NR-17578 |
PUTATIVE ESAT-6 LIKE PROTEIN | MT4024 | Rv3905c | NA |
PUTATIVE ALTERNATIVE RNA POLYMERASE SIGMA FACTOR | MT3431 | Rv3328c | NR-18586 |
PROBABLE METHANOL DEHYDROGENASE REGULATORY PROTEIN | MT3253 | Rv3164c | NR-17574 |
PROBABLE CONSERVED LIPOPROTEIN | MT2865 | Rv2796c | NA |
POSSIBLE OXIDOREDUCTASE | MT3830 | Rv3727 | NR-14960 |
HYPOTHETICAL PROTEIN | MT2291 | Rv* | NR-17577 |
PROBABLE GLYCOGEN PHOSPHORYLASE | MT1370 | Rv1328 | NR-15711 |
HYPOTHETICAL PROTEIN | MT0487 | Rv* | NR-14904 |
PROBABLE FUMARATE REDUCTASE | MT1605 | Rv1554 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT0482 | Rv0466 | NR-15082 |
POSSIBLE CELL DIVISION TRANSMEMBRANE PROTEIN | MT2819 | Rv2748c | NR-17583 |
PROBABLE ALPHA (1 → 2) MANNOSYLTRANSFERASE | MT2236 | Rv2181 | NR-17573 |
1Rv*- In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA- Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到北欧批准. 目标(结核病形成动物研究和基因评估任务性,以使使用定义的方案和测试分枝杆菌的定义突变体(M.Tuberculosis)的定义突变体来实现多种动物物种中的人结核病建模.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突出池8被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池8中的一些转座子突变体可单独使用(参见下面的池8个组分). 每个小瓶中含有大约1ml的中间细菌培养物7h9肉汤,含有100μg/ ml环己酰亚胺的OADC富集和20μg/ ml kanamycin. .customtable {border:0px;单元填充:5px;细胞间距:5px;}.定制TD,TH {边框:0PX;垂直对齐:底部;}#col1 {text-align:left;宽度:70%;}#col2 {text-align:center;宽度:10%;}#col3 {text-align:center;宽度:10%;}#col4 {text-align:center;宽度:10%;} 转座子敲除突变体的描述 菌株 cdc1551 基因 菌株 基因 1 bei资源产品 2 可能脂蛋白 mt1281 RV1244 NA 保守假设蛋白 MT0644 RV0614> NA 可能的铁调节短链脱氢酶/还原酶 mt3321 rv3224 nr-17572 保守假设蛋白 mt3461 Rv3353c na p可挥手氧化还原酶 mt1767 RV1726 NA 可能的保守跨膜蛋白 mt3561 RV3453 可能的两个组分传感器组氨酸激酶 mt3218 Rv3132c nr- 15739 可能保守的整体膜蛋白 mt2795 rv2723 NR-17578 推定的ESAT-6,如蛋白质 MT4024 RV3905C NA 推定的替代RNA聚合酶Sigma因子 mt3431 rv3328c >nr-18586 可能的甲醇脱氢酶调节蛋白质 mt3253 rv3164c 可能保守脂蛋白 mt2865 Rv2796c na 可能的oxidoveryase mt3830 rv3727 nr-14960 假设蛋白 mt2291 RV * >nr-17577 可能糖原磷酸化酶 mt1370 RV1328 假设蛋白 mt0487 RV * 可能的富马酸核苷酸还原酶 mt1605 RV1554 NA 保守假设蛋白 MT0482 rv0466 可能的细胞分裂跨膜蛋白 mt2819 Rv2748c 可能的alpha(1→2)人体脑膜转移酶 mt2236 RV2181 1 rv * - 在某些情况下,没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
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