NR-14829结核分枝杆菌、CDC1551、细胞壁组分(抗原制剂）
产品图片
货号/SKU
NR-14829
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-14829??Mycobacterium tuberculosis, CDC1551, Cell Wall Fraction (Antigen Preparations)|Mycobacterium tuberculosis|CDC1551, Cell Wall Fraction |-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Cell Wall Fraction, NR-14829."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-14829 is a preparation of the cell wall fraction of Mycobacterium tuberculosis (M. tuberculosis), strain CDC1551 and contains proteins and non-protein compounds such as mAGP.
The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4 and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 grams per milliliter in PBS containing 8 mM EDTA, DNase, RNase and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 × g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content and frozen.
Each vial contains approximately 1 mg of cell wall fraction from M. tuberculosis, strain CDC1551 provided in 10 mM ammonium bicarbonate.
NR-14829 is a preparation of the cell wall fraction of Mycobacterium tuberculosis (M. tuberculosis), strain CDC1551 and contains proteins and non-protein compounds such as mAGP.
The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4 and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 grams per milliliter in PBS containing 8 mM EDTA, DNase, RNase and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 × g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content and frozen.
Each vial contains approximately 1 mg of cell wall fraction from M. tuberculosis, strain CDC1551 provided in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-14829是一种制剂的结核分枝杆菌（Cuberculosis），菌株CDC1551，含有蛋白质和非蛋白质化合物如Magp的制备.
将培养物生长至甘油 - 丙氨酸 - 盐培养基中的晚期对数，用PBS pH 7.4洗涤并通过γ辐射灭活.将溶杆菌悬浮在含有8mM EDTA，DNA酶，RNase和蛋白酶抑制剂片的PBS的PBS浓度为每毫升/毫升，并在4℃下在法国压力压力单元中破裂.通过低速（3,000×g）离心除去不间断的细胞.通过以27,000×g离心1小时，将细胞壁分离一小时并在PBS中洗涤两次.将最终的细胞壁沉淀悬浮并在10mM碳酸氢铵中透析，通过BCA蛋白质测定量定量
蛋白质含量和冷冻.
每个小瓶含有约1mg的 m的细胞壁馏分.结核病，菌株CDC1551提供10mM碳酸氢铵.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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