NR-854蓖麻,带有N端组氨酸标签的蓖麻毒素B亚基,来自大肠杆菌的重组体(毒素)
产品图片
货号/SKU
NR-854
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-854??Ricinus communis, Ricin Toxin B Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli(Toxins)|Ricinus communis|Ricin Toxin B Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxin B Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-854."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-854 is a recombinant form of the B chain of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPH) and amino acid residues 315 to 576 of the ricin toxin precursor. A QIAGEN pQE-31 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-854 has a theoretical molecular weight of approximately 30,672 daltons.
The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).
NR-854 reacts specifically with polyclonal antibody to ricin holotoxin and monoclonal antibody to ricin B chain as determined by Western blot analysis.
Each vial of NR-854 contains approximately 1 mg of recombinant ricin toxin B chain suspended in 7 M urea, 25 mM imidazole, and 100 mM sodium phosphate buffer (pH 7.6).
NR-854 is a recombinant form of the B chain of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPH) and amino acid residues 315 to 576 of the ricin toxin precursor. A QIAGEN pQE-31 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-854 has a theoretical molecular weight of approximately 30,672 daltons.
The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).
NR-854 reacts specifically with polyclonal antibody to ricin holotoxin and monoclonal antibody to ricin B chain as determined by Western blot analysis.
Each vial of NR-854 contains approximately 1 mg of recombinant ricin toxin B chain suspended in 7 M urea, 25 mM imidazole, and 100 mM sodium phosphate buffer (pH 7.6).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID批准. NR-854是Ricin毒素的B链的重组形式.氨基酸序列包括蓖麻毒素前体的N-末端组氨酸标签(MRGSHHHHHHHHTP)和氨基酸残基315至576. QIAGEN PQE-31载体用于表达在大肠杆菌中的重组蛋白.通过镍亲和层析纯化蛋白质. NR-854具有大约30,672道尔顿的理论分子量. ricinus communis 基因的序列(genbank:x03179). NR-854特别是通过Western印迹分析测定的Ricin Holotoxin和蓖麻素抗体的多克隆抗体和单克隆抗体对反应的反应. 每个小瓶的NR-854含有约1mg重组蓖麻毒素毒素B.链悬浮在7M尿素,25mM咪唑和100mM磷酸钠缓冲液(pH 7.6)中.
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