NR-53591中东呼吸综合征冠状病毒,来自MERS冠状病毒的刺突糖蛋白(稳定）,英格兰1带有C末端组氨酸和Twin-Strep?标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-53591
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-53591??Middle East Respiratory Syndrome Coronavirus, Spike Glycoprotein (Stabilized) from MERS Coronavirus, England 1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells(Proteins)|Middle East Respiratory Syndrome Coronavirus|Spike Glycoprotein (Stabilized) from MERS Coronavirus, England 1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells| -20°C or colder|DN Sather, P Myler, J McLellanAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from MERS Coronavirus, England 1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells, NR-53591.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from the England 1 strain (UK/H123990006/2012 isolate) of Middle East respiratory syndrome coronavirus (MERS-CoV; GenPept: YP_007188579) was produced by transient transfection into human embryonic kidney HEK293 cells and purified by immobilized metal affinity and gel filtration chromatography. NR-53591 lacks the signal sequence and contains 1274 residues (ectodomain) of the MERS-CoV S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RSVR→ASVG; residues 748 to 751) and VL→PP mutations (residues 1060 and 1061), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? tags. NR-53591 has a theoretical molecular weight of 148,650 daltons. The crystal structure for trimeric S glycoprotein from MERS-CoV has been solved at 4.00 ? resolution (PDB: 5W9P).
The S glycoprotein mediates viral binding to the host dipeptidyl peptidase 4 (DPP4). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-53591 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
A recombinant form of the spike (S) glycoprotein from the England 1 strain (UK/H123990006/2012 isolate) of Middle East respiratory syndrome coronavirus (MERS-CoV; GenPept: YP_007188579) was produced by transient transfection into human embryonic kidney HEK293 cells and purified by immobilized metal affinity and gel filtration chromatography. NR-53591 lacks the signal sequence and contains 1274 residues (ectodomain) of the MERS-CoV S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RSVR→ASVG; residues 748 to 751) and VL→PP mutations (residues 1060 and 1061), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? tags. NR-53591 has a theoretical molecular weight of 148,650 daltons. The crystal structure for trimeric S glycoprotein from MERS-CoV has been solved at 4.00 ? resolution (PDB: 5W9P).
The S glycoprotein mediates viral binding to the host dipeptidyl peptidase 4 (DPP4). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-53591 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自英国1株（英国/ H123990006 / 2012）中东呼吸综合征冠状病毒（MERS-COV; Genpept： YP_007188579 ”是通过瞬态转染在人胚胎肾HEK293细胞中产生的，并通过固定化的金属亲和力和凝胶过滤色谱纯化. NR-53591缺少信号序列，含有MERS-COV S糖蛋白的1274个残基（Ectodomain）;通过在Furin S1 / S2切割位点（RSVR→ASVG;残基748至751）和VL→PP突变（残基1060和1061）稳定重组蛋白，并包括T4 Folcon三聚化结构域，HRV3C蛋白酶切割位点，和C末端Octa-mostatinine和Twin-strep ?标签. NR-53591具有148,650道尔顿的理论分子量.来自MERS-COV的三聚糖蛋白的三聚体S糖蛋白的晶体结构已经在4.00埃分辨率（PDB： 5w9p ）.
S糖蛋白介导病毒结合到宿主二肽基肽酶4（DPP4）.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
每个小瓶在10mM Hepes，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有约100μl的NR-53591.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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