NR-43001重组鼠冠状病毒,icA59-ns2dm(病毒）
产品图片
货号/SKU
NR-43001
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-43001?? Recombinant Murine Coronavirus, icA59-ns2dm(Viruses)|Murine coronavirus|Recombinant Murine Coronavirus, icA59-ns2dm|-60°C or colder|SR WeissAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Recombinant Murine Coronavirus, icA59-ns2dm, NR-43001."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
NR-43001 is a recombinant murine coronavirus that was produced using a vaccinia virus-based reverse genetics system, and derived from cloned, full-length MHV-A59 cDNA. The icA59-ns2dm virus carries two site-directed mutations in the catalytic region of the cyclic phosphodiesterase ns2, resulting in amino acid substitutions at positions 46 (H46A) and 126 (H126R). Viruses carrying either of these two mutations are attenuated for replication in mouse liver following intrahepatic inoculation, but not in mouse brain following intracranial inoculation. The ns2 phosphodiesterase inhibits the interferon-inducible oligoadenylate synthase (OAS)-RNase L pathway by cleaving 2',5'-oligoadenylate, the product of OAS, preventing activation of the cellular endoribonuclease L, thus blocking viral RNA degradation and facilitating hepatitis development.
The complete genome of the recombinant double mutant strain has been sequenced (GenBank: KF268339). In order to remove contaminating mycoplasma, the second viral passage at BEI Resources was performed by lipofectamine-mediated transfection of extracted viral RNA.
Each vial contains approximately 1 mL of cell lysate and supernatant from Mus musculus liver epithelial cells infected with recombinant murine coronavirus, icA59-ns2dm.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
NR-43001 is a recombinant murine coronavirus that was produced using a vaccinia virus-based reverse genetics system, and derived from cloned, full-length MHV-A59 cDNA. The icA59-ns2dm virus carries two site-directed mutations in the catalytic region of the cyclic phosphodiesterase ns2, resulting in amino acid substitutions at positions 46 (H46A) and 126 (H126R). Viruses carrying either of these two mutations are attenuated for replication in mouse liver following intrahepatic inoculation, but not in mouse brain following intracranial inoculation. The ns2 phosphodiesterase inhibits the interferon-inducible oligoadenylate synthase (OAS)-RNase L pathway by cleaving 2',5'-oligoadenylate, the product of OAS, preventing activation of the cellular endoribonuclease L, thus blocking viral RNA degradation and facilitating hepatitis development.
The complete genome of the recombinant double mutant strain has been sequenced (GenBank: KF268339). In order to remove contaminating mycoplasma, the second viral passage at BEI Resources was performed by lipofectamine-mediated transfection of extracted viral RNA.
Each vial contains approximately 1 mL of cell lysate and supernatant from Mus musculus liver epithelial cells infected with recombinant murine coronavirus, icA59-ns2dm.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.这个限制的订单将被发送到NIAID批准.
NR-43001是一种重组鼠冠状病毒，其使用基于痘苗病毒的逆向遗传系统生产，并衍生自克隆，全长MHV-A59 cDNA. ICA59-NS2DM病毒在环状磷酸二酯酶NS2的催化区域中携带两种定向突变，导致位于位置46（H46A）和126（H126R）的氨基酸取代.携带这两个突变中的任何一个的病毒在肝内接种后，在小鼠肝脏中衰减以复制，但在颅内接种后没有小鼠脑. NS2磷酸二酯酶通过切割2'，5'-寡核苷酸，OAS的产物来抑制干扰素诱导的寡核酸合酶（OAS）-LNA族L途径，防止蜂窝状核酸酶L的活化，从而阻断病毒RNA降解并促进肝炎发育.
重组双突变菌株的完整基因组已被测序（GenBank： kf268339 ）.为了去除污染支原体，通过Lipofectamine介导的提取病毒RNA的转染进行了BEI资源的第二病毒通道.
每个小瓶含有大约1ml的细胞裂解物和来自血液的上清液肝上皮细胞感染重组鼠冠状病毒，ICA59-NS2DM.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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