产品描述信息
NR-43938??Human respiratory syncytial virus, A2001/2-20, Purified from HEp-2 Cells(Viruses)|Human respiratory syncytial virus|A2001/2-20, Purified from HEp-2 Cells|-80°C or colder|ML MooreAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Human Respiratory Syncytial Virus, A2001/2-20, Purified From HEp-2 Cells, NR-43938."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
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Human respiratory syncytial virus (RSV), A2001/2-20 was isolated from a nasal wash from an infant with RSV bronchiolitis in Nashville, Tennessee on February 20, 2001.
A2001/2-20 is one of six clinical RSV isolates that recently were shown to induce variable disease severity, lung interleukin-13 (IL-13) levels, and gob-5 levels in BALB/cJ mice. IL-13 is a cytokine linked to mucus production and gob-5 is a calcium-activated chloride channel family member implicated in airway inflammation. Compared to mock infection, RSV A2001/2-20 infection led to relatively high levels of gob-5 and significantly elevated levels of IL-13 in lung tissue. This isolate also induced a bimodal weight loss pattern in infected mice, with peaks at day 2 and day 6 post-infection. RSV A2001/2-20 infection caused the most severe disease of any isolate tested, and was characterized by airway hyperresponsiveness and mucin expression, perivascular edema, epithelial desquamation, bronchiolitis, and increased breathing effort.
NR-43938 was prepared by inoculation of HEp-2 cells (ATCC ? CCL-23?) with RSV, A2001/2-20. The virus was purified from clarified supernatant by high speed centrifugation.
Each vial contains approximately 0.5 mL of NR-43938 in TBS (0.15 M sodium chloride, 0.05 M Tris-HCl, pH 7.6).