NR-727牛痘病毒,改良的安卡拉牛痘(MVA),从BHK-21细胞(病毒)中纯化
产品图片
货号/SKU
NR-727
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-727??Vaccinia virus, Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells(Viruses)|Vaccinia virus|Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells|-70°C or colder|LS Wyatt, B MossAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells, NR-727."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The early passage history of MVA is complex. The complete genomic sequence of MVA has been determined (GenBank: U94848).
MVA is a highly attenuated strain of vaccinia virus and does not appear to replicate in most mammalian cells. Some experts feel MVA can be handled safely by trained personnel without the need for vaccination.
NR-727 was prepared by inoculation of hamster kidney cells (BHK-21) with VACV, MVA. The virus was purified from a crude cell lysate and supernatant by centrifugation through a 36% sucrose cushion.
A sucrose-purified preparation of BHK-21 cells for use as a control is available as BEI Resources NR-794.
Each vial contains approximately 1 mL of NR-727 in TE (pH 7.4).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
The early passage history of MVA is complex. The complete genomic sequence of MVA has been determined (GenBank: U94848).
MVA is a highly attenuated strain of vaccinia virus and does not appear to replicate in most mammalian cells. Some experts feel MVA can be handled safely by trained personnel without the need for vaccination.
NR-727 was prepared by inoculation of hamster kidney cells (BHK-21) with VACV, MVA. The virus was purified from a crude cell lysate and supernatant by centrifugation through a 36% sucrose cushion.
A sucrose-purified preparation of BHK-21 cells for use as a control is available as BEI Resources NR-794.
Each vial contains approximately 1 mL of NR-727 in TE (pH 7.4).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送至NIAID进行批准. MVA的早期段落历史很复杂.已经确定了MVA的完整基因组序列(Genbank:U94848). MVA是一种高度减弱的痘苗病毒菌株,并且在大多数哺乳动物细胞中似乎没有复制.有些专家觉得MVA可以通过培训的人员安全地处理,而无需疫苗接种. 通过接种仓鼠肾细胞(BHK-21)与VACV,MVA进行编写.通过通过36%蔗糖垫离心,从粗细胞裂解物和上清液中纯化病毒. 用于控制的BHK-21细胞的蔗糖纯化的制剂可用作BEI资源NR-794. 每个小瓶在TE(pH 7.4)中包含大约1毫升NR-727. (病毒病原体资源).
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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