NR-55712来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,B.1.621谱系(Mu变体）,带有C末端组氨酸和Avi标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-55712
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55712?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.621 Lineage (Mu Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.621 Lineage (Mu Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells|-20°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.621 Lineage (Mu Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells, NR-55712.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), B.1.621 lineage (Mu variant) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55712 lacks the signal sequence and contains 1197 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55712 includes T95I, insert143T, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H and D950N mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55712 has a theoretical molecular weight of 139,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. B.1.621 is one of several lineages and sublineages designated Mu by the World Health Organization (WHO) and was first identified in Columbia in January 2021. This lineage contains several key mutations of importance to the S glycoprotein: the E484K mutation has been identified in escape mutants from convalescent antisera, and is thought to play a role in the loss of antibody neutralizing activity; N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence; D614G, which is common to the current variants of interest and concern identified by the Centers for Disease Control and Prevention (CDC), was one of the first documented in the U.S. in the initial stages of the pandemic and demonstrates evidence of increasing virus transmissibility; and mutation P681H, part of the S1/S2 proteolytic cleavage site for furin proteases, results in increased cleavage efficiency and may alter antibody recognition sites.
Each vial contains approximately 100 ?L of NR-55712 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), B.1.621 lineage (Mu variant) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55712 lacks the signal sequence and contains 1197 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55712 includes T95I, insert143T, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H and D950N mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55712 has a theoretical molecular weight of 139,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. B.1.621 is one of several lineages and sublineages designated Mu by the World Health Organization (WHO) and was first identified in Columbia in January 2021. This lineage contains several key mutations of importance to the S glycoprotein: the E484K mutation has been identified in escape mutants from convalescent antisera, and is thought to play a role in the loss of antibody neutralizing activity; N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence; D614G, which is common to the current variants of interest and concern identified by the Centers for Disease Control and Prevention (CDC), was one of the first documented in the U.S. in the initial stages of the pandemic and demonstrates evidence of increasing virus transmissibility; and mutation P681H, part of the S1/S2 proteolytic cleavage site for furin proteases, results in increased cleavage efficiency and may alter antibody recognition sites.
Each vial contains approximately 100 ?L of NR-55712 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
在人胚胎肾HEK293细胞中制备了来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），B.1.621谱系（MU变体）的重组形式.通过固定化金属亲和层析纯化. NR-55712缺乏信号序列并含有1197个残基（Ectodomain）的SARS-COV-2 S糖蛋白;通过在Furin S1 / S2切割位点（RrAr→GSAs;残基682至685）和KV→PP突变（残留物986和987;野生型编号），并包括T4 Folcon三聚化结构域，HRV3C，通过取代重组蛋白质蛋白酶切割位点和C末端Octa-组氨酸标签与Avitag?Bira Biotinylation受体序列融合.与SARS-COV-2参考序列（Genpept：）. NR-55712具有139,700道尔顿的理论分子量. SARS-COV-2的三聚糖蛋白的晶体结构已在3.46?分辨率（PDB： 6vsb ）中得到解决.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.这个蛋白质
形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. B.1.621
是世界卫生组织（世卫组织）指定了MU的几个谱系和Sublineages之一，并于2021年1月首次在哥伦比亚确定.该谱系含有几个对S糖蛋白的关键突变：E484K突变已在逃生突变体中鉴定出逃生突变体康复抗苏格拉，
并且被认为在抗体中和活动的丧失中发挥作用; N501Y增加了与ACE2结合的糖蛋白结合，导致SARS-COV-2毒力增加; D614G是疾病控制和预防中心所识别的当前感兴趣的变体和疑虑（CDC）是共同的
在大流行的初始阶段中首次记录在大流行的阶段之一，并证明了增加病毒传播性的证据;和突变p681h，Furin蛋白酶的S1 / S2蛋白水解切割位点的一部分，导致裂解效率增加，并且可以改变抗体识别位点.
每个小瓶在10mM HEPES，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有约100μl的NR-55712.
vipr （病毒病原体资源）提供了附加信息和工具.
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