产品描述信息
NR-3615??Influenza A virus , Kilbourne F130: A/New Jersey/11/1976 (HA, NA) x A/Puerto Rico/8/1934 (H1N1), Large Plaque, Reassortant/Mutant X-53 (CL)-Lp(Viruses)|Influenza A virus|Kilbourne F130: A/New Jersey/11/1976 (HA, NA) x A/Puerto Rico/8/1934 (H1N1), Large Plaque, Reassortant/Mutant X-53 (CL)-Lp|-60°C or colder|NIAID/NIHAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Kilbourne F130: A/New Jersey/11/1976 (HA, NA) x A/Puerto Rico/8/1934 (H1N1), Large Plaque, Reassortant/Mutant X-53 (CL)-Lp, NR-3615."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-3615 is a large plaque mutant isolated after multiple passages of X-53 (CL) (Kilbourne F129; BEI Resources NR-3616) in MDCK cells. X-53 (CL) in turn, was derived by limiting dilution cloning in eggs of X-53 (Kilbourne F128; BEI Resources NR-3664), which is a medium yield A/New Jersey/11/1976 (HA, NA) x A/Puerto Rico/8/1934 (H1N1) reassortant. The HA and NA genes of NR-3615 are definitively derived from influenza A/New Jersey/11/1976 (H1N1) (Kilbourne F9, BEI Resources NR-3595), a human isolate recovered during the 1976 swine flu epidemic at Fort Dix, NJ. All other genes are from A/Puerto Rico/8/1934 (H1N1). The derivation and properties of various A/New Jersey/11/1976 (H1N1) reassortants, as well as cloned derivatives, mutants, and re-reassortants thereof, have been described in detail. The HA of NR-3615 is the low yield (L) variant of A/New Jersey/11/1976 (H1N1). The change from H to L phenotype is associated with a single amino acid change in the HA protein.Each vial contains approximately 1 mL of pooled allantoic fluid from specific pathogen free (SPF) embryonated chicken eggs infected with reassortant/mutant influenza A virus, A/New Jersey/11/1976 (HA, NA) x A/Puerto Rico/8/1934 (H1N1), Large Plaque.
Kilbourne Archive HomepageKilbourne/New York Medical College Archive of Influenza Virus Reassortants and MutantsKilbourne F130 information