NR-40273单克隆抗克里米亚-刚果出血热病毒Pre-Gc糖蛋白,克隆3E3(体外生产)(单克隆抗体）
产品图片
货号/SKU
NR-40273
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-40273?? Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 3E3 (produced in vitro)(Monoclonal Antibodies)|Crimean-Congo hemorrhagic fever virus|Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 3E3 (produced in vitro)|-20°C or colder|CS SchmaljohnAcknowledgment for publications should read "The following reagent was obtained from the Joel M. Dalrymple – Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 3E3 (produced in vitro), NR-40273."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The hybridoma from which NR-40273 was derived is reported to produce antibody of the IgG1 subclass, but NR-40273 occasionally contains both IgG1κ and IgG2bκ probably because of a mixed hybridoma clone. However, the functional activity of this antibody preparation has been confirmed (see below).
Antibody Class: IgG1κ, IgG2bκ
Mouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was purified from clone 3E3 hybridoma supernatant using protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al.
This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.
NR-40273 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV. Although clone 3E3 is reported to lack neutralizing activity against CCHFV, NR-40273 is somewhat active in plaque reduction neutralization tests. Clone 3E3 antibody is also reported to function in ELISA and immunoprecipitation assays, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among several CCHFV strains.
Each vial of NR-40273 contains approximately 100 ?L of purified monoclonal antibody in PBS.
The hybridoma from which NR-40273 was derived is reported to produce antibody of the IgG1 subclass, but NR-40273 occasionally contains both IgG1κ and IgG2bκ probably because of a mixed hybridoma clone. However, the functional activity of this antibody preparation has been confirmed (see below).
Antibody Class: IgG1κ, IgG2bκ
Mouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was purified from clone 3E3 hybridoma supernatant using protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al.
This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.
NR-40273 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV. Although clone 3E3 is reported to lack neutralizing activity against CCHFV, NR-40273 is somewhat active in plaque reduction neutralization tests. Clone 3E3 antibody is also reported to function in ELISA and immunoprecipitation assays, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among several CCHFV strains.
Each vial of NR-40273 contains approximately 100 ?L of purified monoclonal antibody in PBS.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
据报道，衍生NR-40273的杂交瘤以产生IgG1亚类的抗体，但是NR-40273偶尔含有IgG1κ和IgG2Bκ可能，可能是因为混合的杂交瘤克隆.然而，已经证实了该抗体制剂的功能活性（见下文）.
抗体类：IgG1κ，IgG2Bκ
使用蛋白G亲和色谱法从克隆3e3杂交瘤上清液中纯化针对克里米诺刚果出血热病毒（CCHFV）前GC糖蛋白的小鼠单克隆抗体.通过用蛋白质A琼脂糖结合的CCHFV糖蛋白 - 抗体复合物免疫的BALB / C小鼠的SP2 / 0-AG14小鼠小鼠骨髓瘤细胞的融合产生B细胞杂交瘤.
该试剂是Joel M. Dalrymple的一部分 - Clarence J. Peters Usamrid抗体收集.
NR-40273使用CCHFV感染的Vero E6细胞在间接免疫荧光测定中是反应性的.虽然据报道克隆3E3缺乏对CCHFV的中和活性，但NR-40273在斑块还原测试中有些活跃.还报道克隆3E3抗体在ELISA和免疫沉淀测定中起作用，以部分保护来自致死的CCHFV攻击的乳胶小鼠，并识别在几种CCHFV菌株中保守的表位.
每个小瓶NR-40273在PBS中含有约100μl纯化的单克隆抗体.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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