- 14 次围观
产品图片

产品别名
货号/SKU
EUI007
货号/规格
120ug
库存与交货期
1-2周
人民币价格
8785
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of James H. Morrissey, PhD, University of Michigan.
产品描述信息
Product Type: | Small Molecule |
Name: | Polyphosphate, Medium Chain (p100; biotinylated) |
Chemical Formula: | (NaPO3)n[actual counterions may be Na &/or Li; nis approximately 45 to 160] |
Molecular Weight: | Heterogeneous; Number average ~11.5 kDa; Range ~2 kDa to 28.5 kDa |
Format: | Lyophilized |
Purity: | |
Buffer: | 42 mM Tris-HCl pH 8.5, 208 mM NaCl, 4.2 mM EDTA |
Solubility: | At least 100 mM in water; |
Concentration: | When reconstituted:10 mM (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length |
Size Distribution: | Mode of n: 75 (as measured by migration on PAGE) |
Comments: | It is recommended to use ~10.2 ug of the biotin-polyphosphate to coat all wells of a streptavidin-coated 96-well plate. |
Storage: |
Dry material should be stored at room temperature (or lower) with dessication. Once reconstituted: - Stable at least 12 hours at room temperature - Stable at least 72 hours at 4C - Store long-term at -80C - Aliquot for repeated use, avoiding repeated freeze-thaw cycles - Do not store in the presence of divalent metal ions |
Shipped: | Ambient temperature |
产品安全信息
Morrissey JH, Choi SH, and Smith S.A. Polyphosphate: an ancient molecule that links platelets, coagulation and inflammation. Blood 119:5972-5979, 2012.Rao NN, Gómez-García MR, and Kornberg A. Inorganic polyphosphate: essential for growth and survival. Annu Rev Biochem. 78:605-647, 2009.Dinarvand P, Hassanian SM, Qureshi SH, Manithody C, Eissenberg JC, Yang L, Rezaie AR. Polyphosphate amplifies proinflammatory responses of nuclear proteins through interaction with receptor for advanced glycation end products and P2Y1 purinergic receptor. Blood. 2014 Feb 6;123(6):935-45.Suess PM, Gomer RH. Extracellular polyphosphate inhibits proliferation in anautocrine negative feedback loop in Dictyostelium discoideum. J Biol Chem. 2016 Aug 12. pii: jbc.M116.737825. View ArticleMorrissey JH. Poly-P as Modulator of Hemostasis, Thrombosis, and Inflammation. Blood 2017 130:SCI-1.Bentley-DeSousa A, Holinier C, Moteshareie H, Tseng YC, Kajjo S, Nwosu C, Amodeo GF, Bondy-Chorney E, Sai Y, Rudner A, Golshani A, Davey NE, Downey M. A Screen for Candidate Targets of Lysine Polyphosphorylation Uncovers a Conserved Network Implicated in Ribosome Biogenesis. Cell Rep. 2018 Mar 27;22(13):3427-3439. View ArticleLorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Life Sci Alliance. 2019 May 27;2(3). pii: e201900385. View ArticleSuess PM, Chinea LE, Pilling D, Gomer RH. Extracellular Polyphosphate Promotes Macrophage and Fibrocyte Differentiation, Inhibits Leukocyte Proliferation, and Acts as a Chemotactic Agent for Neutrophils. J Immunol. 2019 Jul 15;203(2):493-499. View articleNegreiros RS, Lander N, Huang G, Cordeiro CD, Smith SA, Morrissey JH, Docampo R. Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids. Mol Microbiol. 2018 Dec;110(6):973-994.View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
该多磷酸盐(中链,生物素化)的尺寸是非均相的,具有近似聚合物长度范围为〜45-160次磷酸盐单元;模态尺寸约为75个磷酸盐单位。高灯非常相似的血小板分泌的天然多磷酸盐的尺寸分布含有共价连接到的生物素,可以固定在三相磷酸酯链的末端磷酸盐上,可以固定在链霉素蛋白涂覆的多孔板或珠子上用于结合研究的珠子多磷酸盐聚磷酸盐的蛋白质,在生物体中普遍存在的无机正磷酸盐的线性聚合物,并且在生物学中起着不同的作用,最近被证明是人血液凝固系统的有效调制器。其促凝血活性依赖于聚合物长度。这种生物素化的中链多磷酸盐(P100)的尺寸分布非常相似,与血小板分泌的天然多磷酸盐。除了作为等离子体凝血测定的有用工具,可以使用多磷酸盐来研究细菌生长,毒力和细菌膜功能。可用于:多磷酸盐,中链(P100)多磷酸,长链(P700)生物素化多磷酸盐,长链(P700)来自密歇根大学詹姆斯H.Morrissey实验室,博士学位。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识

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