- 15 次围观
产品图片
产品别名
货号/SKU
ESK201-FP
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
13585
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
产品描述信息
Product Type: | Cell Line |
Name: | L6 |
Cell Type: | Skeletal muscle |
Organism: | Rat |
Accession ID: | CVCL_0385 |
Source: | Quadriceps |
Morphology: | Myoblast/Myotube |
Biosafety Level: | II |
Subculturing: | 2-3 days |
Growth Conditions: | MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS (See protocol below) |
Cryopreservation: | MEM-α +10% FBS+ 10% DMSO |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
产品安全信息
Cell Line ReferencesMitsumoto Y, Burdett E, Grant A, Klip A. (1991) Differential expression of the GLUT1 and GLUT4 glucose transporters during differentiation of L6 muscle cells. Biochem Biophys Res Commun. 175: 652-9.Tsakiridis T, Vranic M, Klip A. (1994) Disassembly of the Actin Network Inhibits Insulin-dependent Stimulation of Glucose Transport and Prevents Recruitment of Glucose Transporters to the Plasma Membrane. J. Biol. Chem. 269: 29934-42.Huang C, Somwar R, Patel N, Niu W, Török D, Klip A. Sustained exposure of L6 myotubes to high glucose and insulin decreases insulin-stimulated GLUT4 translocation but upregulates GLUT4 activity. Diabetes. 2002 Jul;51(7):2090-8.Application ReferencesPillon NJ, Li YE, Fink LN, Brozinick JT, Nikolayev A, Kuo MS, Bilan PJ, KlipA. (2014) Nucleotides released from palmitate-challenged muscle cells through pannexin-3 attract monocytes. Diabetes. 63: 3815-26.Li Q, Zhu X, Ishikura S, Zhang D, Gao J, Sun Y, Contreras-Ferrat A, Foley KP, Lavandero S, Yao Z, Bilan PJ, Klip A, Niu W. (2014) Ca²? signals promote GLUT4 exocytosis and reduce its endocytosis in muscle cells. Am J Physiol Endocrinol Metab. Jul 15;307(2):E209-24.Sun Y, Chiu TT, Foley KP, Bilan PJ, Klip A. (2014) Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells. Mol Biol Cell. 25: 1159-70.Foley KP, Klip A. (2014) Dynamic GLUT4 sorting through a syntaxin-6 compartment in muscle cells is derailed by insulin resistance-causing ceramide. Biol Open. 3: 314-25.Chiu TT, Sun Y, Koshkina A, Klip A. (2013) Rac-1 superactivation triggers insulin-independent glucose transporter 4 (GLUT4) translocation that bypasses signaling defects exerted by c-Jun N-terminal kinase (JNK)- and ceramide-induced insulin resistance. J Biol Chem. 288:17520-31.Pillon NJ, Arane K, Bilan PJ, Chiu TT, Klip A. (2012) Muscle cells challenged with saturated fatty acids mount an autonomous inflammatory response that activates macrophages. Cell Commun Signal. 10: 30.Boguslavsky S, Chiu T, Foley KP, Osorio-Fuentealba C, Antonescu CN, Bayer KU, Bilan PJ, Klip A. (2012) Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles. Mol Biol Cell. 23: 4065-78.Kewalramani G, Fink LN, Asadi F, Klip A. (2011) Palmitate-activated macrophages confer insulin resistance to muscle cells by a mechanism involving protein kinase C ? and ?. PLoS One. 6: e26947.Sun Y, Bilan PJ, Liu Z, Klip A. (2010) Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells. Proc Natl Acad Sci U S A. 107: 19909-14.Ishikura S, Antonescu CN, Klip A. (2010) Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers. Curr. Protoc. Cell Biol., John Wiley & Sons, Inc., 46: Unit 15.15: 1-9.Samokhvalov V, Bilan PJ, Schertzer JD, Antonescu CN, Klip A. (2009) Palmitate- and lipopolysaccharide-activated macrophages evoke contrasting insulin responses in muscle cells. Am J Physiol Endocrinol Metab. 296: E37-46.Antonescu CN, Díaz M, Femia G, Planas JV, Klip A. (2008) Clathrin-dependent and independent endocytosis of glucose transporter 4 (GLUT4) in myoblasts: regulation by mitochondrial uncoupling. Traffic. 9: 1173-90.Thong FS, Bilan PJ, Klip A. (2007) The Rab GTPase-activating protein AS160 integrates Akt, protein kinase C, and AMP-activated protein kinase signals regulating GLUT4 traffic. Diabetes. 56: 414-23.Wijesekara N, Tung A, Thong F, Klip A. (2006) Muscle cell depolarization induces again in surface GLUT4 via reduced endocytosis independently of AMPK. Am J Physiol Endocrinol Metab. 290: E1276-86.Ishiki M, Randhawa VK, Poon V, Jebailey L, Klip A. (2005) Insulin regulates the membrane arrival, fusion, and C-terminal unmasking of glucose transporter-4 via distinct phosphoinositides. J Biol Chem. 280: 28792-802.Rudich A, Konrad D, Török D, Ben-Romano R, Huang C, Niu W, Garg RR, Wijesekara N, Germinario RJ, Bilan PJ, Klip A. (2003) Indinavir uncovers different contributions of GLUT4 and GLUT1 towards glucose uptake in muscle and fat cells and tissues. Diabetologia 46: 649-58.Wang Q, Somwar R, Bilan PJ, Liu Z, Jin J, Woodgett JR, Klip A. (1999) Protein kinase B/Akt participates in GLUT4 translocation by insulin in L6 myoblasts. Mol Cell Biol. 19: 4008-18.Wang Q, Khayat Z, Kishi K, Ebina Y, Klip A. (1998) GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Letters 427: 193-7.Jaldin-Fincati JR, Bilan PJ, Klip A. GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence. Methods Mol Biol. 2018;1713:175-192. View ArticleKalinovich A, Dehvari N, Åslund A, et al. Treatment with a β-2-adrenoceptor agonist stimulates glucose uptake in skeletal muscle and improves glucose homeostasis, insulin resistance and hepatic steatosis in mice with diet-induced obesity [published online ahead of print, 2020 May 29]. Diabetologia. 2020;10.1007/s00125-020-05171-y.View ArticleKalinovich A, Dehvari N, Åslund A, et al. Treatment with a β-2-adrenoceptor agonist stimulates glucose uptake in skeletal muscle and improves glucose homeostasis, insulin resistance and hepatic steatosis in mice with diet-induced obesity. Diabetologia. 2020;63(8):1603-1615. View ArticleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
未加工的大鼠骨骼(L6)肌细胞细胞系,用于高融合电位和肌室中的Glut4中的内源性表达。高灯:将高可靠性分化为肌室肌细胞表型,天然表达Glut4葡萄糖转运蛋白显着的胰岛素刺激葡萄糖摄取生物反应可用于新的抗糖尿病复合筛选可用于通过基于质粒的基因转移和病毒感染方案(逆转录病毒和腺病毒)胰岛素刺激骨骼肌的胰岛素刺激主要是由于葡萄糖转运蛋白的易位的血糖输送到细胞表面。 L6细胞最初来自大鼠骨骼肌,作为单核肌细胞繁殖,但可以分化为多核原发性肌管。 Myotubes表达了几种典型的骨骼肌,包括Glut4葡萄糖转运蛋白。胰岛素刺激具有高灵敏度和最大反应性的葡萄糖摄取,并且在分化的L6 myotubes和Glut4表达方中,随着L6细胞分化,可以获得这些特性。 L6 myotubes的这些特征是重要的,因为Glut4负责成熟的骨骼肌中的胰岛素依赖性葡萄糖摄取。还有:L6-Glut4MYC大鼠肌细胞肌细胞系列来自Amira Klip,Phd,Sick Childs医院的实验室。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
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