- 54 次围观
产品图片
产品别名
货号/SKU
EH1011
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
10855
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
产品描述信息
Product Type: | Cell Line |
Name: | BHK-21 |
Cell Type: | Baby Hamster Kidney |
Organism: | Mesocricetus auratus(hamster, Syrian golden) |
Source: | Kidney |
Morphology: | Epithelial/Fibroblast |
Biosafety Level: | Bsl-1 |
Growth Conditions: | DMEM + 5% FBS; 37oC, 6-8% CO2 |
Subculturing: | Passage 1:5-1:3 every day, or 1:20 every 2-3 days. |
Cryopreservation: | 70% DMEM; 20% FBS; 10% DMSO |
Comments: | For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74. |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
产品安全信息
Helenius, A., and H. Soderlund. 1973. Stepwise dissociation of the Semliki Forest virus membrane with Triton X-100. Biochem, Biophys.Acta 307:287-300.Jayakar, H. R., K. G. Murti, and M. A. Whitt. 2000. Mutations in the PPPY motif of vesicular stomatitis virus matrix protein reduce virus budding by inhibiting a late step in virion release. J. Virol 74:9818-9827.Vaheri A, Sedwick WD, Plotkin SA, Maes R. Cytopathic effect of rubella virus in BHK21 cells and growth to high titers in suspension culture. Virology. 1965 Oct;27(2):239-41.Kaarianen, L., K. Simons, and C.-H. von Bonsdorff. 1969. Studies in subviral components of Semliki Forest virus. Ann.Med.Exp.Fenn 47:235-248.Lawson, N. D., E. A. Stillman, M. A. Whitt, and J. K. Rose. 1995. Recombinant vesicular stomatitis viruses from DNA. Proc.Natl.Acad.Sci.(USA) 92:4477-4481.Macpherson, I., and M. Stoker. 1962. Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147-151.Whitt, M. A. 2010. Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods 169:365-74.Suarez MA, Valencia J, Cadena CC, Maiti R, Datta C, Puerto G, Isaza JH, San Juan H, Nagaraja V, Guzman JD. Diarylethenes Display In Vitro Anti-TB Activity and Are Efficient Hits Targeting the Mycobacterium tuberculosis HU Protein. Molecules. 2017 Jul 25;22(8). pii: E1245. View ArticleZeng Z, Yu H, Chen H, Qi W, Chen L, Chen G, Yan W, Chen T, Ning Q, Han M, Wu D. Longitudinal changes of inflammatory parameters and their correlation with disease severity and outcomes in patients with COVID-19 from Wuhan, China. Crit Care. 2020 Aug 27;24(1):525.View articleGalindo I, Garaigorta U, Lasala F, Cuesta-Geijo MA, Bueno P, Gil C, Delgado R, Gastaminza P, Alonso C. Antiviral drugs targeting endosomal membrane proteins inhibit distant animal and human pathogenic viruses. Antiviral Res. 2020 Nov 26:104990.View articleHashem AM, Algaissi A, Almahboub SA, Alfaleh MA, Abujamel TS, Alamri SS, Alluhaybi KA, Hobani HI, AlHarbi RH, Alsulaiman RM, ElAssouli MZ, Hala S, Alharbi NK, Alhabbab RY, AlSaieedi AA, Abdulaal WH, Bukhari A, Al-Somali AA, Alofi FS, Khogeer AA, Pain A, Alkayyal AA, Almontashiri NAM, Ahmad BM, Li X. Early Humoral Response Correlates with Disease Severity and Outcomes in COVID-19 Patients. Viruses. 2020 Dec 4;12(12):E1390.View articleAlmahboub SA, Algaissi A, Alfaleh MA, ElAssouli MZ, Hashem AM. Evaluation of Neutralizing Antibodies Against Highly Pathogenic Coronaviruses: A Detailed Protocol for a Rapid Evaluation of Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudovirus-Based Assay. Front Microbiol. 2020 Sep 4;11:2020.View ArticleFarzani TA, Chov A, Herschhorn A. A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses. STAR Protoc. 2020 Dec 9;1(3):100209. View articleWu K, Werner AP, Moliva JI, Koch M, Choi A, Stewart-Jones GBE, Bennett H, Boyoglu-Barnum S, Shi W, Graham BS, Carfi A, Corbett KS, Seder RA, Edwards DK. mRNA-1273 vaccine induces neutralizing antibodies against spike mutants from global SARS-CoV-2 variants. bioRxiv [Preprint]. 2021 Jan 25:2021.01.25.427948. View articleBaggen J, Persoons L, Vanstreels E, Jansen S, Van Looveren D, Boeckx B, Geudens V, De Man J, Jochmans D, Wauters J, Wauters E, Vanaudenaerde BM, Lambrechts D, Neyts J, Dallmeier K, Thibaut HJ, Jacquemyn M, Maes P, Daelemans D. Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2. Nat Genet. 2021 Apr;53(4):435-444. View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
这些BHK-21细胞是高度透气的,用于回收最初由Lawson等人(5)所述的传染性重组囊肿口炎病毒(RVSV),并由Jayakar等(2)改性,或用于复制和扩增复制 - 限制ΔG-VSV,如Whitt(7)所述。细胞的特征在于同工酶分析,证实细胞来自叙利亚金仓鼠。使用策略支原体PCR引物凝固,细胞也已被证明是没有的支原体。从迈克尔A. Whitt,Ph.D.,田纳西大学的实验室。部分调查员的附件计划。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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