NR-53762_SARS相关冠状病毒2,USA-WA1/2020重组传染性分子克隆质粒试剂盒(质粒/载体）

NR-53762?? SARS-Related Coronavirus 2, USA-WA1/2020 Recombinant Infectious Molecular Clone Plasmid Kit(Plasmid/Vectors)|SARS-Related Coronavirus 2|SARS-Related Coronavirus 2, USA-WA1/2020 Recombinant Infectious Molecular Clone Plasmid Kit| -20°C or colder|RS BaricAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, USA-WA1/2020 Recombinant Infectious Molecular Clone Plasmid Kit, NR-53762.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Note: Infectious viral particles produced by use of this kit are a BSL3 organism. Virus production should be performed with appropriate biosafety controls. Note: The vial labels for NR-53752 to NR-53758 indicate these products are a molecular clone in vector pU57. The correct vector is pUC57 and each plasmid produces a viral fragment that must be combined with additional fragments to produce the molecular clone. The NR-53755 label also lists this clone as wildtype (WT); however, NR-53755 is not WT and includes a T15102A silent mutation. The vectors for the recombinant infectious molecular clone from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), USA-WA1/2020 (GenBank: MT461669) was designed by RT-PCR amplification of SARS-CoV-2 virus (GenBank: MT020880) with restriction sites and four-nucleotide cohesive ends at the 5’ and 3’ insert termini and subcloned into the pUC57 expression vector. NR-53762 can be used to assemble recombinant infectious SARS-CoV-2, USA-WA/2020 and consists of the eight plasmids listed in Table 1. Descriptions of each component are included below.

Table 1: SARS-CoV-2 Molecular Clone Plasmid Kit

Plasmid Type	Insert	BEI ResourcesCatalog Number
Virus fragment	cDNA fragment A	NR-53752
Virus fragment	cDNA fragment B	NR-53753
Virus fragment	cDNA fragment C	NR-53754
Virus fragment	cDNA fragment D	NR-53755
Virus fragment	cDNA fragment E	NR-53756
Virus fragment	cDNA fragment F	NR-53757
Virus fragment	cDNA fragment G	NR-53758
Helper Plasmid	sgRNA-N	NR-53761

All plasmids contain the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The complete plasmid sequences and maps are provided on the BEI Resources webpage. The plasmids were produced in E. coli and extracted. Each kit contains one vial of each plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. The vials should be centrifuged prior to opening. Note: The contents of each vial should be used to replicate the plasmid in E. coli prior to mammalian expression.