产品图片
货号/SKU
NR-52425
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52425?? Vector pMCSG53 Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Non-Structural Protein 10 Gene(Plasmid/Vectors)|SARS-Related Coronavirus 2|Vector pMCSG53 Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Non-Structural Protein 10 Gene| -20°C or colder|KJ SatchellAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pMCSG53 Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Non-Structural Protein 10 Gene, NR-52425, contributed by the Center for Structural Genomics of Infectious Diseases under HHSN272201700060C.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The non-structural protein 10 (nsp10) gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was codon optimized and cloned into the pMCSG53 plasmid. pMCSG53 is an Escherichia coli (E. coli) expression vector that contains an N-terminal hexa-histidine tag, followed by a tobacco etch virus (TEV) protease recognition site prior to the insert coding sequence, resulting in the expression of a cleavable histidine-tagged protein. It also contains tRNA genes covering rare codons for Arg (AGG/AGA) and Ile (AUA) to improve expression in E. coli. The beta-lactamase gene, bla, provides transformant selection through ampicillin resistance in E. coli. The resulting size of the plasmid is approximately 5230 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
Non-structural protein 10 is a non-structural protein located within the SARS-CoV-2 ORF1ab and is required for viral replication. It has been shown to stimulate the activity of two methyl transferases, Nsp14 and Nsp16, resulting in the production of a viral RNA cap that prevents the activation of the host innate immune response.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to expression studies.
The non-structural protein 10 (nsp10) gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was codon optimized and cloned into the pMCSG53 plasmid. pMCSG53 is an Escherichia coli (E. coli) expression vector that contains an N-terminal hexa-histidine tag, followed by a tobacco etch virus (TEV) protease recognition site prior to the insert coding sequence, resulting in the expression of a cleavable histidine-tagged protein. It also contains tRNA genes covering rare codons for Arg (AGG/AGA) and Ile (AUA) to improve expression in E. coli. The beta-lactamase gene, bla, provides transformant selection through ampicillin resistance in E. coli. The resulting size of the plasmid is approximately 5230 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
Non-structural protein 10 is a non-structural protein located within the SARS-CoV-2 ORF1ab and is required for viral replication. It has been shown to stimulate the activity of two methyl transferases, Nsp14 and Nsp16, resulting in the production of a viral RNA cap that prevents the activation of the host innate immune response.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to expression studies.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的非结构蛋白10（NSP10）基因，武汉-U-1（Genbank：结果908947 ）是密码子优化和克隆到PMCSG53质粒中. PMCSG53是含有N-末端六胞组氨酸标签的大肠杆菌（大肠杆菌）表达载体，其次是在插入编码序列之前的烟草蚀刻病毒（TEV）蛋白酶识别部位，得到在可切割的组氨酸标记蛋白的表达中.它还含有TRNA基因，覆盖罕见的arg（agg / aga）和Ile（aua）的稀有密码子，以改善在 e中的表达. Coli . β-内酰胺酶基因， BLA 通过在 e中通过氨苄青霉素抗性提供转化体选择. Coli .得到的质粒尺寸约为5230碱基对.在北部资源网页上提供完整的质粒序列和地图.在 e中制备质粒. Coli 并提取.
非结构蛋白10是位于SARS-COV-2 ORF1ab内的非结构蛋白，并且是病毒复制所必需的.已经证明刺激两种甲基转移酶的活性，NSP14和NSP16，导致生病毒RNA帽的产生，可防止主体先天免疫反应的激活.
每个小瓶在Te缓冲液中含有质粒DNA（10mM Tris-HCl，1mM EDTA，pH8.0）.在打开之前应该离心小瓶. 注意：Vial的内容应该用于复制 e中的质粒. Coli 在表达研究之前.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2021-12-21
2024-12-25
2022-04-01
2022-04-01
2024-08-19
2021-10-31
2022-08-11
2021-12-21
2022-04-01
2022-04-01
2022-04-01
2024-01-21
2024-05-30
2021-12-21
2021-12-21