NR-52350_定量PCR(qPCR)从热灭活的SARS相关冠状病毒2中提取对照,分离物USA-WA1/2020(灭活生物体）

NR-52350?? Quantitative PCR (qPCR) Extraction Control from Heat-Inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020(Inactivated Organisms)|SARS-Related Coronavirus 2|Quantitative PCR (qPCR) Extraction Control from Heat-Inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020| -60°C or colder |CDCAcknowledgment for publications should read "The following reagent was deposited by the Centers for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH: Quantitative PCR (qPCR) Extraction Control from Heat-Inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020, NR-52350."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-52350 contains a preparation of heat-inactivated severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA-WA1/2020 (BEI Resources lot 70048020) diluted into Homo sapiens lung carcinoma cells (A549; ATCC ^? CCL-185?), for use as an extraction control in qPCR assays. The source material is a preparation of SARS-CoV-2, isolate USA-WA1/2020 that has been inactivated by heating to 65°C for 30 minutes. Each vial contains approximately 250 ?L of heat-inactivated SARS-CoV-2 and A549 cells, in phosphate-buffered saline (PBS). The vial should be centrifuged prior to opening.