NR-50532_来自3型登革热病毒的基因组RNA,DENV-3/US/BID-V1043/2006(核酸）

NR-50532?? Genomic RNA from Dengue Virus Type 3, DENV-3/US/BID-V1043/2006(Nucleic Acids)|Dengue virus type 3|Genomic RNA from Dengue Virus Type 3, DENV-3/US/BID-V1043/2006|-60°C or colder|JL Munoz-JordanAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic RNA From Dengue Virus Type 3, DENV-3/US/BID-V1043/2006, NR-50532."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic RNA was isolated from a preparation of cell lysate and supernatant from Aedes albopictus mosquito larval epithelial cells (clone C6/36; ATCC ^? CRL-1660?) infected with dengue virus type 3 (DEN-3), DENV-3/US/BID-V1043/2006. DEN-3, DENV-3/US/BID-V1043/2006 was isolated from a human in Puerto Rico in 2006. The complete genome of DEN-3, DENV-3/US/BID-V1043/2006 has been sequenced (GenBank: EU482555). NR-50532 has been qualified for RT-PCR applications by amplification of a sequence of approximately 1000 nucleotides. Each vial contains approximately 100 ?L of genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7). The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.