Sci Rep. 2025 Dec 29. doi: 10.1038/s41598-025-33355-2. Online ahead of print.
ABSTRACT
A small multiple-dose regimen of cyclophosphamide (CY) compared to a large single dose in prepubertal mice has shown reduced follicle loss and improved fertility outcomes at early reproductive age. However, the long-term effects on the competency of oocytes from such surviving follicles, compared to those at post-pubertal age, remain unknown. Small multiple CY (75 mg/Kg, four weekly doses) injections were administered to prepubertal (PP75X4) and adolescent (AD75X4) Swiss albino female mice. At 22 weeks of age, surviving females were assessed for follicular pool and oocyte functional competency. The resulting embryos were assessed for blastulation, total cell number (TCN) quality, and in vitro inner cell mass (ICM) progression. At 22 weeks of life, PP75X4 females had a three-fold reduction (p
PMID:41461837 | DOI:10.1038/s41598-025-33355-2