NR-50115单克隆抗甲型流感病毒N8神经氨酸酶(NA),克隆1G10(体外生产)(单克隆抗体）

NR-50115?? Monoclonal Anti-Influenza A Virus N8 Neuraminidase (NA), Clone 1G10 (produced in vitro)(Monoclonal Antibodies)|Influenza A virus|Monoclonal Anti-Influenza A Virus N8 Neuraminidase (NA), Clone 1G10 (produced in vitro)|-20°C or colder|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Influenza A Virus N8 Neuraminidase (NA), Clone 1G10 (produced in vitro), NR-50115."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Antibody Class: IgG2aκMouse monoclonal antibody prepared against the N8 neuraminidase (NA) protein of influenza A virus was purified from clone 1G10 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with splenocytes from BALB/c mice immunized by sequential intranasal infection with live influenza virus A/mallard/Interior Alaska/10BM01929/2010 (H10N7) and reassortant influenza virus A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8), followed by boosting with poly-I:C-adjuvanted, formalin-inactivated A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8).Each vial of NR-50115 contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline, pH 7.4.NR-50115 recognizes influenza A virus A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8)-infected cells in indirect immunofluorescence assays, and neutralizes A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8) in vitro. The clone 1G10 antibody is also reported to be active in neuraminidase inhibition (NI) assays, and to exhibit some cross-reactivity with recombinant N8 NA of the Eurasian Lineage in ELISA.