NR-50090_来自查菲埃里希氏菌的基因组DNA,菌株St.Vincent(核酸）

NR-50090?? Genomic DNA from Ehrlichia chaffeensis, Strain St. Vincent(Nucleic Acids)|Ehrlichia chaffeensis|Genomic DNA from Ehrlichia chaffeensis, Strain St. Vincent|-20°C or colder|Y Rikihisa, Centers for Disease Control and PreventionAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Ehrlichia chaffeensis, Strain St. Vincent, NR-50090."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was isolated from a preparation of cell lysate and supernatant from Canis familiaris macrophage-monocyte cells (DH82; ATCC ^? CRL-10389?) infected with Ehrlichia chaffeensis (E. chaffeensis), strain St. Vincent. E. chaffeensis, strain St. Vincent was isolated in 1996 from the blood of a 52-year-old male patient in Georgia, USA, who was bitten by a tick and subsequently developed fatal human monocytic ehrlichiosis (HME). The complete genome sequence of E. chaffeensis, strain St. Vincent has been determined (GenBank: CP007478). NR-50090 has been qualified for PCR applications by amplification of approximately 1000 base pairs of the ECH_0849 gene. Each vial contains 100 ?L of bacterial genomic DNA. The bacterial genomic DNA is in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8.0) in a background of cellular nucleic acid (lot 70021819) or TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.0) in a background of cellular nucleic acid and carrier RNA (lot 63958928). The vial should be centrifuged prior to opening.