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Light Sci Appl. 2025 Aug 12;14(1):272. doi: 10.1038/s41377-025-01949-0.
ABSTRACT
Increased micro- and nanoplastic (MNP) pollution poses significant health risks, yet the mechanisms of their accumulation and effects on absorptive tissues remain poorly understood. Addressing this knowledge gap requires tractable models coupled to dynamic live cell imaging methods, enabling multi-parameter single cell analysis. We report a new method combining adult stem cell-derived small intestinal organoid cultures with live fluorescence lifetime imaging microscopy (FLIM) to study MNP interactions with gut epithelium. To facilitate this, we optimized live imaging of porcine and mouse small intestinal organoids with an 'apical-out' topology. Subsequently, we produced a set of pristine MNPs based on PMMA and PS (
PMID:40796554 | PMC:PMC12343940 | DOI:10.1038/s41377-025-01949-0