NR-44223来自鼠疫耶尔森氏菌的组分1荚膜抗原(F1),在大肠杆菌中表达(蛋白质）

NR-44223 is a recombinant form of the Yersinia pestis (Y. pestis) fraction 1 capsular antigen (F1). F1 is a plasmid (pFra)-encoded proteinaceous capsule synthesized in large quantities by Y. pestis and reported to confer antiphagocytic properties on the pathogen by interfering with complement-mediated opsonization. The protein is highly immunogenic and has been indirectly associated with eliciting a protective immune response in humans. A recombinant protein lacking the native signal sequence but including an N-terminal hexa-histidine tag, a thrombin cleavage site, and amino acid residues 22 to 170 of F1 from Y. pestis (GenPept: AAS58714) was expressed in Escherichia coli and purified by nickel affinity chromatography. Treatment with immobilized bovine thrombin resulted in a protein with four non-F1 residues at the amino terminus. The thrombin-cleaved protein was further purified by anion exchange chromatography and another round of nickel affinity chromatography to remove the cleaved hexa-histidine tag. NR-44223 has a theoretical molecular weight of 15,976 daltons.

Each vial contains approximately 200 ?g of NR-44223 in phosphate-buffered saline, pH 7.4.