- 4 次围观
产品图片
货号/SKU
NR-15787
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15787??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 15(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 15|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 15, NR-15787."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 15 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 15 are available individually (see Pool 15 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 15 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 15 are available individually (see Pool 15 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
POSSIBLE 3-METHYLADENINE DNA GLYCOSYLASE MPG | MT727.1 | Rv1688 | NR-15056 |
POSSIBLE RNA METHYLTRANSFERASE | MT2178 | Rv2118c | NA |
CONSERVED HYPOTHETICAL PROTEIN WITH PIN DOMAIN | MT3492 | Rv3384c | NR-15065 |
PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE | MT1980 | Rv1928c | NR-18583 |
CONSERVED HYPOTHETICAL PROTEIN MEMBER OF YABO/YCEC/YFII FAMILY | MT1592 | Rv1540 | NA |
PROBABLE MEMBRANE BOUND POLYKETIDE SYNTHASE PKS6 | MT0418 | Rv0405 | NR-18244 |
HYPOTHETICAL PROTEIN | MT2405 | Rv* | NR-18252 |
PROBABLE OXIDOREDUCTASE | MT0074 | Rv0068 | NA |
PROBABLE BIFUNCTIONAL NUCLEOSIDASE | MT0100 | Rv0091 | NA |
PROBABLE TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT | MT3015 | Rv2943 | NR-18406 |
PROBABLE O-METHYLTRANSFERASE OMT | MT1187 | Rv1153c | NA |
PROBABLE COBALAMIN BIOSYNTHESIS PROTEIN COBN | MT2121 | Rv2062c | NA |
POSSIBLE CONSERVED TRANSMEMBRANE PROTEIN | MT0534 | Rv0513 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT1550 | Rv1501 | NA |
HYPOTHETICAL PROTEIN | MT3974.1 | Rv* | NA |
PPE FAMILY PROTEIN | MT1406 | Rv1361c | NA |
CONSERVED HYPOTHETICAL TRANSMEMBRANE PROTEIN | MT1567 | Rv1517 | NA |
PPE FAMILY PROTEIN | MT3636 | Rv3532 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT1354 | Rv1314c | NA |
PPE FAMILY PROTEIN | MT0168 | Rv0159c | NA |
1Rv*- In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA- Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到北欧批准. 目标(结核病形成动物研究和基因评估任务性),以使使用定义的方案和测试定义的 m的定义突变体来实现多种动物物种中的人结核病的建模.结核病.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突出池15被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池15中的一些转座子突变体可单独使用(参见下面的池15个组分). 每个小瓶中含有大约1毫升含有100μg/ ml环己酰亚胺的OADC富集的1ml细菌培养物和20μg/ ml kanamycin. 组件: 转座子敲除突变体的描述 菌株 cDc1551 基因 菌株 H37RV 基因 1 bei资源 产品 2 可能的3-甲基腺嘌呤DNA糖基酶MPG MT727.1 rv1688 NR-15056 可能的RNA甲基转移酶 MT2178 RV2118C na 带有针域的保守假设蛋白质 mt34 92 rv3384c NR-15065 可能短链型脱氢酶/还原酶 MT1980 RV1928C nr-18583 yabo / ycec / yfii家族的保守假设蛋白质成员 mt1592 rv1540 na 可能膜结合聚酮合成酶PKS6 Mt0418 RV0405 假设蛋白 mt2405 rv * 可能的oxidoaductase mt0074 rv0068 na 可能的双官能核苷酶 mt0100 RV0091 Na 用于插入序列元件的可能转座酶 RV2943 nr-18406 可能的O-甲基转移酶OMT MT1187 RV1153C Na 可能的钴胺蛋白生物合成蛋白COBN MT2121 RV2062C Na 可能的保守跨膜蛋白 mt0534 rv0513 na 保守假设蛋白 MT1550 RV1501 Na 假设蛋白 MT3974.1 RV * Na PPE家族蛋白 MT1406 RV1361C na 保守假设跨膜蛋白 mt1567 RV1517 Na PPE家族蛋白 Mt3636 RV3532 Na 保守假设蛋白 mt1354 rv1314c na PPE家族蛋白 Mt0168 RV0159C Na 1 rv * - 在某些情况下没有 m .结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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