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产品图片
货号/SKU
NR-15508
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-15508?? Vaccinia Virus, Western Reserve, Recombinant Expressing Whitewater Arroyo Virus, AV 9310135 Glycoprotein Precursor(Viruses)|Whitewater Arroyo Virus|Vaccinia Virus, Western Reserve, Recombinant Expressing Whitewater Arroyo Virus, AV 9310135 Glycoprotein Precursor|-60°C or colder|MJ BuchmeierAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve, Recombinant Expressing Whitewater Arroyo Virus, AV 9310135 Glycoprotein Precursor, NR-15508."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A cDNA clone containing the entire ORF encoding the glycoprotein precursor (GPC) from the S segment of Whitewater Arroyo virus (WWAV), AV 9310135 was inserted into the pRB21 transfer vector, bringing it under the control of a synthetic vaccinia virus (VACV) early/late promoter (PSYN). Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV strain vRB12.
WWAV is an Arenavirus (Arenaviridae, Arenavirus) which has been tentatively associated with hemorrhagic fever and acute respiratory distress syndrome, acute central nervous system disease, and undifferentiated febrile illness in humans in the United States.
Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ? CRL-2761?) infected with vaccinia virus, rVACV-WWAV GPC.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A cDNA clone containing the entire ORF encoding the glycoprotein precursor (GPC) from the S segment of Whitewater Arroyo virus (WWAV), AV 9310135 was inserted into the pRB21 transfer vector, bringing it under the control of a synthetic vaccinia virus (VACV) early/late promoter (PSYN). Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV strain vRB12.
WWAV is an Arenavirus (Arenaviridae, Arenavirus) which has been tentatively associated with hemorrhagic fever and acute respiratory distress syndrome, acute central nervous system disease, and undifferentiated febrile illness in humans in the United States.
Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ? CRL-2761?) infected with vaccinia virus, rVACV-WWAV GPC.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此项目每年可订购两次.通过此限制的订单将在发货前发送到NIAID. 从白水Arroyo病毒(WWAV)的S段中包含整个ORF的CDNA克隆,其中包含糖蛋白前体(GPC),将AV 9310135插入PRB21转移载体中,使其在合成痘苗病毒(ACV)早期/晚期启动子(PSYN)的控制下.通过将转移质粒转染到用VRB12感染的CV-1细胞中来制备重组捕获.这一直暂时与出血热和急性呼吸窘迫综合征,急性中枢神经系统疾病和美国人类的初期患有无分化的发热疾病相关. 每个小瓶含有大约1毫升细胞裂解物和来自 Cercopithecus Aethiops 肾上皮细胞(BSC-40,ATCC ? CRL-2761?)感染痘苗病毒,Rvacv-Wwav GPC. 其他信息和工具可在 vipr (病毒病原体资源).
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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