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产品图片
货号/SKU
NR-14824
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-14824??Mycobacterium tuberculosis, HN878, Whole Cell Lysate(Antigen Preparations)|Mycobacterium tuberculosis|HN878, Whole Cell Lysate|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain HN878, Whole Cell Lysate, NR-14824."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mycobacterium tuberculosis, strain HN878 whole cell lysate contains proteins, lipids and carbohydrates present within the bacterial cell.
A culture was grown to late-log phase in glycerol-alanine-salts medium, and inactivated by gamma irradiation. Cells were suspended in PBS buffer containing 8 mM EDTA, proteinase inhibitors, DNase, and RNase and disrupted by French Press until approximately 90% breakage was obtained. The lysate was centrifuged to pellet the unbroken cells, and the cleared supernatant was removed. The protein content of the whole cell lysate was quantified using the BCA protein assay.
Each vial of whole cell lysate contains approximately 10 mg of protein in 10 mM ammonium bicarbonate provided as a frozen pellet.
Mycobacterium tuberculosis, strain HN878 whole cell lysate contains proteins, lipids and carbohydrates present within the bacterial cell.
A culture was grown to late-log phase in glycerol-alanine-salts medium, and inactivated by gamma irradiation. Cells were suspended in PBS buffer containing 8 mM EDTA, proteinase inhibitors, DNase, and RNase and disrupted by French Press until approximately 90% breakage was obtained. The lysate was centrifuged to pellet the unbroken cells, and the cleared supernatant was removed. The protein content of the whole cell lysate was quantified using the BCA protein assay.
Each vial of whole cell lysate contains approximately 10 mg of protein in 10 mM ammonium bicarbonate provided as a frozen pellet.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
结核分枝杆菌,菌株HN878全细胞裂解物含有细菌细胞内存在的蛋白质,脂质和碳水化合物.
将培养物生长至甘油 - 丙氨酸 - 盐培养基中的后对数相,并通过γ辐射灭活.将细胞悬浮在含有8mM EDTA,蛋白酶抑制剂,DNA酶和RNase的PBS缓冲液中,并通过法式压机破坏直至获得约90%的断裂.将裂解物离心以沉淀不间断的细胞,除去清除的上清液.使用BCA蛋白质测定定量全细胞裂解物的蛋白质含量.
每个小瓶的全细胞裂解物在10mM碳酸氢盐中含有约10mg蛋白质,作为冷冻沉淀.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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