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产品图片
货号/SKU
NR-10501
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-10501?? Variola Major Virus (Bangladesh-1975) M1R Protein, Recombinant from Baculovirus (Homolog of Vaccinia Virus L1R)(Proteins)|Variola major virus|Variola Major Virus (Bangladesh-1975) M1R Protein, Recombinant from Baculovirus (Homolog of Vaccinia Virus L1R)|-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Variola Major Virus (Bangladesh-1975) M1R Protein, Recombinant from Baculovirus, NR-10501."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-10501 is a recombinant form of the variola major (Bangladesh-1975) M1R protein, a homolog of the vaccinia virus (WR) L1R protein. The full-length variola major virus M1R protein contains 250 amino acid residues (GenPept: AAA60821; GenBank: L22579). NR-10501 is a truncated form of M1R, comprising amino acid residues 1-185, and lacking the C-terminal transmembrane domain of the intact protein. NR-10501 was produced by baculovirus infection of Trichoplusia ni insect larvae using the proprietary Chesapeake PERL technology, PERLXpress. The protein was purified using standard chromatographic methods.
NR-10501 was demonstrated to be functionally active based on its reactivity with an anti-vaccinia (WR) L1R (residues 1 to 185) mouse monoclonal antibody (VMC-2 provided by G. H. Cohen and R. J. Eisenberg; monoclonal antibody prepared from the same hybridoma as VMC-2 is available as BEI Resources NR-417).
Each vial contains approximately 1 mg of NR-10501 in 30 mM phosphate buffer (pH 7.6) containing 50 mM KCl, 100 mM NaCl and 0.05% polysorbate (v/v).
NR-10501 is a recombinant form of the variola major (Bangladesh-1975) M1R protein, a homolog of the vaccinia virus (WR) L1R protein. The full-length variola major virus M1R protein contains 250 amino acid residues (GenPept: AAA60821; GenBank: L22579). NR-10501 is a truncated form of M1R, comprising amino acid residues 1-185, and lacking the C-terminal transmembrane domain of the intact protein. NR-10501 was produced by baculovirus infection of Trichoplusia ni insect larvae using the proprietary Chesapeake PERL technology, PERLXpress. The protein was purified using standard chromatographic methods.
NR-10501 was demonstrated to be functionally active based on its reactivity with an anti-vaccinia (WR) L1R (residues 1 to 185) mouse monoclonal antibody (VMC-2 provided by G. H. Cohen and R. J. Eisenberg; monoclonal antibody prepared from the same hybridoma as VMC-2 is available as BEI Resources NR-417).
Each vial contains approximately 1 mg of NR-10501 in 30 mM phosphate buffer (pH 7.6) containing 50 mM KCl, 100 mM NaCl and 0.05% polysorbate (v/v).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-10501是variola主要(Bangladesh-1975)M1R蛋白的重组形式,疫苗病毒的同源物( WR)L1R蛋白.全长Variola主要病毒M1R蛋白含有250个氨基酸残基(Genpept:AAA60821; Genbank:L22579). NR-10501是M1R的截短形式,包含氨基酸残基1-185,并缺乏完整蛋白质的C末端跨膜结构域. NR-10501是通过使用专有的Chesapeake Perl技术,Perlxpress的 Trichoplusia Ni 昆虫幼虫产生的NR-10501.使用标准色谱法纯化蛋白质. NR-10501基于与抗疫苗(WR)L1R(残基1至185)小鼠单克隆抗体(GMC-2提供的VMC-2和RJ艾森伯提供的VMC-2;单克隆与VMC-2相同的杂交瘤制备的抗体可作为BEI资源NR-417获得). 每个小瓶在含有50mM KCl,100mM NaCl和0.05%聚山梨醇酯(V / v)的30mM磷酸盐缓冲液(pH7.6)中含有大约1mg -10501.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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