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产品图片
货号/SKU
NR-10303
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-10303?? Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA1346(Nucleic Acids)|Bacillus anthracis|Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA1346|-20°C or colder|P HannaAcknowledgment for publications should read "The following reagent was contributed by P. Hanna, University of Michigan for distribution through BEI Resources, NIAID, NIH: Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA1346, NR-10303."|Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was isolated from a preparation of Bacillus anthracis (B. anthracis), strain Sterne ΔGBAA1346.
B. anthracis, strain Sterne ΔGBAA1346 is a markerless, nonpolar, deletion mutant of the toxigenic acapsulate original Sterne strain (34F2). Nearly the entire open reading frame is replaced by three stop codons followed by two restriction endonuclease recognition sites, BamHI and SmaI (to facilitate screening of the correct mutation). The first and last ten codons of the putative internalin (GBAA1346) gene retain the wild type sequence. Additional information is available at the Resource Center for Biodefense Proteomics Research (BPRC).
NR-10303 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. The presence of plasmid pX01 and absence of plasmid pX02 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Each vial contains 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).
Genomic DNA was isolated from a preparation of Bacillus anthracis (B. anthracis), strain Sterne ΔGBAA1346.
B. anthracis, strain Sterne ΔGBAA1346 is a markerless, nonpolar, deletion mutant of the toxigenic acapsulate original Sterne strain (34F2). Nearly the entire open reading frame is replaced by three stop codons followed by two restriction endonuclease recognition sites, BamHI and SmaI (to facilitate screening of the correct mutation). The first and last ten codons of the putative internalin (GBAA1346) gene retain the wild type sequence. Additional information is available at the Resource Center for Biodefense Proteomics Research (BPRC).
NR-10303 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. The presence of plasmid pX01 and absence of plasmid pX02 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Each vial contains 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).
主要内容
此项目的数量限制为1.此商品可以每年订购一次.此限制的订单将在发货前发送到NIAID进行批准. 基因组DNA从A>杆菌（B.炭疽）的制备中分离出来（B.炭疽），菌株SterneΔGBAA1346 . b.蒽氏菌，菌株替补ΔGBAA1346是无标，非极性，毒性酸纤维氏菌菌株（34F2）的无标记缺失突变体.几乎整个开放阅读框架由三个止挡密码子取代，然后是两个限制性内切核酸酶识别位点， Bam Hi和 Sma I（以便于筛选正确的突变）.推定的内蛋白（GBAA1346）基因的第一个和最后十个密码子保留野生型序列. 资源中心对于生物义蛋白质组学研究（BPRC）. NR-10303通过扩增16S核糖体RNA基因的约1500bp，NR-10303已获得PCR应用.通过来自提取的DNA的质粒特异性序列的PCR扩增证实了质粒pX01和不存在质粒PX02. 每个小瓶在TE缓冲液中含有4至6μg的细菌基因组DNA（10 mm Tris- HCl，1mM EDTA，pH?7.4）.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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