产品图片
货号/SKU
MRA-57
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-57?? Plasmodium falciparum yPfs28C Protein, Recombinant from Saccharomyces cerevisiae, Strain VK1(Proteins)|Plasmodium falciparum|Plasmodium falciparum yPfs28C Protein, Recombinant from Saccharomyces cerevisiae, Strain VK1|-70°C|DC KaslowAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum yPfs28C Protein, Recombinant from Saccharomyces cerevisiae, Strain VK1, MRA-57, contributed by David C. Kaslow."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Yeast-secreted recombinant 28 kDa sexual-stage surface antigen from Plasmodium falciparum (P. falciparum). The open reading frame of Pfs28 from P. falciparum was expressed as a recombinant protein in Saccharomyces cerevisiae, strain VK1 using expression plasmid pIXY154. The DNA sequence encoding Pfs28 comes from Val24 to Ser196 and contains a carboxy-terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).
Each vial of MRA-57 contains approximately 100 ?L of purified recombinant P. falciparum Pfs28C in phosphate-buffered saline (pH 7.4).
Protein function: antigen
Tissue/cellular location: sexual-stage surface
Gene coding for protein: Pfs28
Purification methods: NI-NTA purification
Quality control methods: SDS-PAGE
Gozar, M. M., V. L. Price and D. C. Kaslow. "Saccharomyces cerevisiae-Secreted Fusion Proteins Pfs25 and Pfs28 Elicit Potent Plasmodium falciparum Transmission-Blocking Antibodies in Mice." Infect. Immun. 66 (1998): 59-64. PubMed: 9423839.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Yeast-secreted recombinant 28 kDa sexual-stage surface antigen from Plasmodium falciparum (P. falciparum). The open reading frame of Pfs28 from P. falciparum was expressed as a recombinant protein in Saccharomyces cerevisiae, strain VK1 using expression plasmid pIXY154. The DNA sequence encoding Pfs28 comes from Val24 to Ser196 and contains a carboxy-terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).
Each vial of MRA-57 contains approximately 100 ?L of purified recombinant P. falciparum Pfs28C in phosphate-buffered saline (pH 7.4).
Protein function: antigen
Tissue/cellular location: sexual-stage surface
Gene coding for protein: Pfs28
Purification methods: NI-NTA purification
Quality control methods: SDS-PAGE
Gozar, M. M., V. L. Price and D. C. Kaslow. "Saccharomyces cerevisiae-Secreted Fusion Proteins Pfs25 and Pfs28 Elicit Potent Plasmodium falciparum Transmission-Blocking Antibodies in Mice." Infect. Immun. 66 (1998): 59-64. PubMed: 9423839.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到北欧以批准. 该试剂由贡献者认证. Bei资源尚未确认或验证了这种材料. 酵母分泌的重组28kDa性阶段表面抗原来自疟原虫(p. falciparum). PFS28的开放阅读框架来自 p. Falciparum 在酿酒酵母中表示为重组蛋白,菌株VK1使用表达质粒pixy154.编码PFS28的DNA序列来自Val24至Ser196,包含羧基端子His6标签.蛋白质分泌并从培养上清液中恢复.使用微滤,切向超滤渗滤和尺寸排阻色谱(Superdex 75)纯化蛋白质. 每个小瓶的MRA-57含有大约100μl纯化的重组1.磷酸盐缓冲盐水中的恶性脂饼(pH7.4). 蛋白质功能:抗原组织/细胞位置:性阶段表面基因编码蛋白质:PFS28 纯化方法:Ni-NTA净化质量控制方法:SDS-PAGE Gozar,MM,VL价格和DC KASLOW. “ Saccharomyces酿酒酵母 - 分泌融合蛋白PFS25和PFS28引出效率疟原虫疟原虫在小鼠中传输阻断抗体.” 感染. IMMUN. 66(1998):59-64. PubMed:9423839.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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