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产品图片
货号/SKU
MRA-178
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-178?? Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium vivax(Plasmid/Vectors)|Plasmodium vivax|Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium vivax|-20°C or colder|PA ZimmermanAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium vivax, MRA-178, contributed by Peter A. Zimmerman.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
MRA-178 is an Escherichia coli (E. coli) plasmid encoding the small subunit ribosomal RNA gene (18S rRNA gene; GenBank: AF145335) from Plasmodium vivax (P. vivax), strain Thai 3. The small subunit ribosomal RNA gene was amplified from genomic DNA by nest 1 PCR primers and cloned into vector pCR2.1-TOPO (Invitrogen?). The resulting plasmid (clone 16) may be used in PCR assays for the diagnosis of mixed species malaria infections. The recommended host for expression is E. coli TOP10. A multiple cloning site flanks the 18S rRNA open reading frame. Ampicillin and kanamycin (E. coli) were incorporated as selectable markers.
The resulting size of the plasmid is approximately 5000 base pairs.
Each vial contains approximately 0.5 ?g of plasmid DNA in buffer. The vial should be centrifuged prior to opening.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
MRA-178 is an Escherichia coli (E. coli) plasmid encoding the small subunit ribosomal RNA gene (18S rRNA gene; GenBank: AF145335) from Plasmodium vivax (P. vivax), strain Thai 3. The small subunit ribosomal RNA gene was amplified from genomic DNA by nest 1 PCR primers and cloned into vector pCR2.1-TOPO (Invitrogen?). The resulting plasmid (clone 16) may be used in PCR assays for the diagnosis of mixed species malaria infections. The recommended host for expression is E. coli TOP10. A multiple cloning site flanks the 18S rRNA open reading frame. Ampicillin and kanamycin (E. coli) were incorporated as selectable markers.
The resulting size of the plasmid is approximately 5000 base pairs.
Each vial contains approximately 0.5 ?g of plasmid DNA in buffer. The vial should be centrifuged prior to opening.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
MRA-178是编码小亚基核糖体RNA基因的大肠杆菌(大肠杆菌)(大肠杆菌)(大肠杆菌)质粒(18s rRNA基因; Genbank: af145335 ”从疟原虫(p.vivax),菌株泰国3.巢1 pcr引物从基因组DNA扩增小亚基核糖体RNA基因并克隆到载体PCR2.1-TOPO(Invitrogen ?)中.得到的质粒(克隆16)可用于PCR测定以诊断混合物种疟疾感染.用于表达的推荐主机是 e. COLI TOP10.多克隆部位侧翼到18s rRNA开放阅读框架.氨苄西林和卡那霉素(例如COLI)被用作可选标记.
质粒的所得尺寸约为5000碱基对.
每个小瓶在缓冲液中含有大约0.5μg的质粒DNA.在打开之前应该离心小瓶.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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