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产品图片
货号/SKU
ARP-2852
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
ARP-2852??Toxoplasma gondii, Toxoplasma gondii Fusion Protein Expression Vector (pminP30/G), Recombinantin Escherichia coli(Plasmid/Vectors)|Toxoplasma gondii|Toxoplasma gondii Fusion Protein Expression Vector (pminP30/G), Recombinant
in Escherichia coli|-60°C or colder?|DS Roos, EO KendallAcknowledgment for publications should read “The following reagent was provided by the NIH AIDS Reagent Program for distribution by BEI Resources, NIAID, NIH: Toxoplasma gondii Fusion Protein Expression Vector (pminP30/G), Recombinant in Escherichia coli, ARP-2852, contributed by Dr. David S. Roos."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-2852 is an Escherichia coli (E. coli) DH5a stock containing a fusion protein vector expressing the initiation codon and signal sequence from the Toxoplasma gondii (T. gondii) major surface protein P30 (SAG1) fused to the rabies virus glycoprotein (G). The P30 sequence was amplified from genomic DNA by PCR. The flanking sequences were derived from the T. gondii dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. A BgIII site separates the 5’ untranslated region (UTR) from the P30 coding sequence, and a PstI site separates the G coding sequencing from the 3’ UTR. The construct was cloned into Bluescript pKS+ (Stratagene) from EcoRv to SmaI, during which both sites were destroyed.
ARP-2852 is useful for construction of P30 fusion proteins for expression in transfected parasites. Although expression of rabies virus G-protein is very poor, the construct is suitable for replacing the G-protein gene with other transgenes of interest.
Each vial contains approximately 500 ?L of E. coli DH5a with pminP30/G in Luria Bertani (LB) broth supplemented with 10% glycerol.
ARP-2852 is an Escherichia coli (E. coli) DH5a stock containing a fusion protein vector expressing the initiation codon and signal sequence from the Toxoplasma gondii (T. gondii) major surface protein P30 (SAG1) fused to the rabies virus glycoprotein (G). The P30 sequence was amplified from genomic DNA by PCR. The flanking sequences were derived from the T. gondii dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. A BgIII site separates the 5’ untranslated region (UTR) from the P30 coding sequence, and a PstI site separates the G coding sequencing from the 3’ UTR. The construct was cloned into Bluescript pKS+ (Stratagene) from EcoRv to SmaI, during which both sites were destroyed.
ARP-2852 is useful for construction of P30 fusion proteins for expression in transfected parasites. Although expression of rabies virus G-protein is very poor, the construct is suitable for replacing the G-protein gene with other transgenes of interest.
Each vial contains approximately 500 ?L of E. coli DH5a with pminP30/G in Luria Bertani (LB) broth supplemented with 10% glycerol.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. ARP-2852
是一个大肠杆菌 (例如coli) dh5a股票含有融合
表达引发密码子和来自弓形虫的信号序列的蛋白质载体主要
与狂犬病病毒糖蛋白(g)融合的表面蛋白质p30(sag1).从基因组中扩增p30序列
DNA通过PCR.侧翼序列是
来自 T. Gondii 二氢酚酸还原酶 - 胸苷晶酯合成酶(DHFR-TS)基因. A BGI II
站点将5'未转换的区域(UTR)与P30编码序列分开,
和 pst i网站将g分开
从3'UTR编码测序.这
将构建体克隆到从 Eco RV到 SMA I的BlueScript PKS +(stratagene),在此期间两个网站被破坏. ARP-2852对于构建P30融合蛋白的表达是有用的
转染寄生虫.虽然
狂犬病病毒G蛋白的表达非常差,构建体是合适的
用其他目的的其他转基因取代G蛋白基因. 每个小瓶包含大约500μl的 e.大肠杆菌 DH5A在LURIA的PMINP30 / g
Bertani(LB)肉汤补充有10%甘油.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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