NR-431牛痘病毒,单克隆抗牛痘(WR)B5R,残基20至275,胞外域(类似于VMC-16),(体外生产)(单克隆抗体)
产品图片
货号/SKU
NR-431
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-431??Vaccinia virus, Monoclonal Anti-Vaccinia (WR) B5R, Residues 20 to 275, Ectodomain (similar to VMC-16), (produced in vitro) (Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia (WR) B5R, Residues 20 to 275, Ectodomain (similar to VMC-16), (produced in vitro) |-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) B5R Protein, Residues 20 to 275 (Ectodomain), (similar to VMC-16), (produced in vitro), NR-431."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mouse monoclonal antibody to a recombinant form of the B5R envelope glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-431 was purified from the same hybridoma as VMC-16. The specificity of VMC-16 was determined by reactivity to B5R(275t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 20 to 275 of B5R indicates that VMC-16 recognizes epitopes within amino acids 56 to 75 and 254 to 264. VMC-16 does not neutralize the infectivity of the extracellular enveloped virus (EEV) form of vaccinia virus in BS-C-1 cells using an EEV plaque reduction assay. VMC-16 does not inhibit the comet tail formation of the EEV form of vaccinia virus in BS-C-1 cells using a comet tail inhibition assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
Mouse monoclonal antibody to a recombinant form of the B5R envelope glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-431 was purified from the same hybridoma as VMC-16. The specificity of VMC-16 was determined by reactivity to B5R(275t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 20 to 275 of B5R indicates that VMC-16 recognizes epitopes within amino acids 56 to 75 and 254 to 264. VMC-16 does not neutralize the infectivity of the extracellular enveloped virus (EEV) form of vaccinia virus in BS-C-1 cells using an EEV plaque reduction assay. VMC-16 does not inhibit the comet tail formation of the EEV form of vaccinia virus in BS-C-1 cells using a comet tail inhibition assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID以进行批准. 每个小瓶在50毫米中含有约1.0ml纯化的单克隆抗体硼酸盐缓冲液(pH 8.0 +/- 0.2)含有0.1米氯化钠,无防腐剂.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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