NR-51211单克隆抗痘苗病毒(WR)B5R蛋白,残基20至275,(胞外域)(类似于VMC-14),(体外生产)(单克隆抗体)
产品图片
货号/SKU
NR-51211
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-51211?? Monoclonal Anti-Vaccinia Virus (WR) B5R Protein, Residues 20 to 275, (Ectodomain) (Similar to VMC-14), (produced in vitro)(Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia Virus (WR) B5R Protein, Residues 20 to 275, (Ectodomain) (Similar to VMC-14), (produced in vitro)|-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) B5R Protein, Residues 20 to 275 (Ectodomain), (similar to VMC-14), (produced in vitro), NR-51211.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG1κ
Mouse monoclonal antibody to a recombinant form of the B5R envelope glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes.
Each vial contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline (PBS).
NR-51211 was purified from the same hybridoma as VMC-14. The specificity of VMC-14 was determined by reactivity to B5R(275t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions.The reactivity pattern in ELISA assays using overlapping peptides spanning residues 20 to 275 of B5R indicates that VMC-14 recognizes an epitope within amino acids 56 to 75. VMC-14 does not neutralize the infectivity of the extracellular enveloped virus (EEV) form of vaccinia virus in BSC-1 cells using an EEV plaque reduction assay. VMC-14 inhibits the comet tail formation of the EEV form of vaccinia virus in BSC-1 cells using a comet tail inhibition assay.
Antibody Class: IgG1κ
Mouse monoclonal antibody to a recombinant form of the B5R envelope glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes.
Each vial contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline (PBS).
NR-51211 was purified from the same hybridoma as VMC-14. The specificity of VMC-14 was determined by reactivity to B5R(275t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions.The reactivity pattern in ELISA assays using overlapping peptides spanning residues 20 to 275 of B5R indicates that VMC-14 recognizes an epitope within amino acids 56 to 75. VMC-14 does not neutralize the infectivity of the extracellular enveloped virus (EEV) form of vaccinia virus in BSC-1 cells using an EEV plaque reduction assay. VMC-14 inhibits the comet tail formation of the EEV form of vaccinia virus in BSC-1 cells using a comet tail inhibition assay.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到NIAID批准. 抗体类:IgG1κ
小鼠单克隆
B5R封套糖蛋白的重组形式的抗体[B5R(275t);
残留物20至275包含胞外域,N-末端组氨酸标记的]
西方储备(WR)痘苗病毒菌株纯化
杂交瘤上清液通过蛋白G亲和色谱法. b细胞杂交瘤由该细胞产生
用免疫BALB / C脾细胞融合SP2 / 0骨髓瘤细胞. 每个小瓶含有约100μl
纯化的磷酸盐缓冲盐水(PBS)的单克隆抗体. 从同一杂交瘤纯化NR-51211
作为VMC-14. VMC-14的特异性是
由ELISA的反应性确定于B5R(275T)并通过Western印迹证实
减少和非降低条件下的分析.ELISA测定中的反应性模式
重叠肽跨越B5R的残基20至275表示VMC-14
识别氨基酸56至75中的表位.VMC-14不中和感染率
BSC-1细胞中的细胞外包膜病毒(EEV)痘苗病毒的形式
使用EEV斑块减少测定. VMC-14.
在BSC-1中抑制EEV痘苗病毒的EEV形式的彗星尾部形成
细胞使用彗星尾抑制测定.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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