NR-10307_来自炭疽杆菌的基因组DNA,StrainSterneΔGBAA0599-2(核酸）
产品图片
货号/SKU
NR-10307
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-10307?? Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA0599-2(Nucleic Acids)|Bacillus anthracis|Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA0599-2|-20°C or colder|P HannaAcknowledgment for publications should read "The following reagent was contributed by P. Hanna, University of Michigan for distribution through BEI Resources, NIAID, NIH: Genomic DNA from Bacillus anthracis, Strain Sterne ΔGBAA0599-2, NR-10307."|Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was isolated from a preparation of Bacillus anthracis (B. anthracis), strain Sterne ΔGBAA0599-2.
B. anthracis, strain Sterne ΔGBAA0599-2 is a deletion mutant of the toxigenic acapsulate original Sterne strain (34F2), constructed by replacing codons 10 through 14 with three in-frame stop codons followed by the recognition site for BamHI (to facilitate screening of the correct mutation). The remainder of the neutral protease gene (nprB; GBAA0599-2) retains the wild type sequence. Additional information is available at the Resource Center for Biodefense Proteomics Research (BPRC).
NR-10307 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. The presence of plasmid pX01 and absence of plasmid pX02 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Each vial contains 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4).
Genomic DNA was isolated from a preparation of Bacillus anthracis (B. anthracis), strain Sterne ΔGBAA0599-2.
B. anthracis, strain Sterne ΔGBAA0599-2 is a deletion mutant of the toxigenic acapsulate original Sterne strain (34F2), constructed by replacing codons 10 through 14 with three in-frame stop codons followed by the recognition site for BamHI (to facilitate screening of the correct mutation). The remainder of the neutral protease gene (nprB; GBAA0599-2) retains the wild type sequence. Additional information is available at the Resource Center for Biodefense Proteomics Research (BPRC).
NR-10307 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. The presence of plasmid pX01 and absence of plasmid pX02 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Each vial contains 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4).
主要内容
此项目的数量限制为1.此商品可以每年订购一次.此限制的订单将在发货前发送至NIAID进行批准. 基因组DNA从制剂中分离杆菌（B.炭疽），菌株SterneΔGBAA0599 -2. b.蒽氏菌氏菌菌氏菌δGBAA0599-2是通过用三个内框架停止密码子更换密码子10到14而构成的毒性碎屑原始斯托纳菌株（34F2）的缺失突变体，其次是用于 BAM的识别部位 hi（以促进筛选正确的突变）.中性蛋白酶基因的其余部分（ NprB ; GBAA0599-2）保留野生型序列. 资源中心对于生物义蛋白质组学研究（BPRC）. 通过扩增16s核糖体RNA基因的约1500bp，NR-10307已获得PCR应用.通过来自提取的DNA的质粒特异性序列的PCR扩增证实了质粒pX01和不存在质粒PX02. 每个小瓶在TE缓冲液中含有4至6μg的细菌基因组DNA（10 mm Tris- HCl和1 mm EDTA，pH?7.4）.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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