Smart Tube样品裂解液

250 milliliter bottle of Proteomic Stabilizer PROT1. Sufficient to stabilize over 170 milliliters of whole blood when used as recommended. Also includes buffer used after thawing samples stored frozen in Stabilizer: THAWLYSE1 "Thaw-Lyse, 1000X Concentrate".  Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351692 Protocol for banking samples with PROT1 Protocol for thawing samples banked with PROT1 Published antibody panels used to analyze samples banked in PROT1 Published Protocols and Methods Papers using PROT1 Smets, Tina, et al. "Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)." Multiple Myeloma. Humana Press, New York, NY, 2018. 47-54. Behbehani, Gregory K. "Cell Cycle Analysis by Mass Cytometry." Cellular Quiescence. Humana Press, New York, NY, 2018. 105-124. Fernandez, Rosemary, and Holden Maecker. "Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry." Bio-protocol 5.11 (2015).   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Geanon, Daniel, et al. "A Streamlined CyTOF Workflow To Facilitate Standardized Multi-Site Immune Profiling of COVID-19 Patients." medRxiv 2020.06.26.20141341 (2020). Rahil, Zainab, et al. "Landscape of coordinated immune responses to H1N1 challenge in humans." Journal of Clinical Investigation, Vol. 130 (2020). Brandt, Raphael, et al. "Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium." Nature Communications, Vol. 10, Article 2919 (2019). Tsai, Amy, et al. "A year-long immune profile of the systemic response in acute stroke survivors." Brain, Vol. 142, Iss. 4 (2019). Chen, Luxi, et al. "CD56 Expression Marks Human Group 2 Innate Lymphoid Cell Divergence from a Shared NK Cell and Group 3 Innate Lymphoid Cell Developmental Pathway." Immunity (2018).  Ajami, Bahareh, et al. "Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models." Nature Neuroscience 21.4 (2018): 541. Spitzer, Matthew H., et al. "Systemic immunity is required for effective cancer immunotherapy." Cell 168.3 (2017): 487-502. Glassberg, Jeffrey, et al. "Application of phospho-CyTOF to characterize immune activation in patients with sickle cell disease in an ex vivo model of thrombosis." Journal of immunological methods 453 (2018): 11-19. Blazkova, Jana, et al. "Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy." The Journal of Immunology (2017): 1601855. Schulz, Axel Ronald, et al. "Silver nanoparticles for the detection of cell surface antigens in mass cytometry." Cytometry Part A 91.1 (2017): 25-33. Ciarlo, Eleonora, et al. "Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo." Scientific reports 6 (2016): 37944. Fragiadakis, Gabriela K., et al. "Patient-specific immune states before surgery are strong correlates of surgical recovery." Anesthesiology: The Journal of the American Society of Anesthesiologists 123.6 (2015): 1241-1255. Spitzer, Matthew H., et al. "An interactive reference framework for modeling a dynamic immune system." Science 349.6244 (2015): 1259425. Behbehani, Gregory K., et al. "Mass cytometric functional profiling of acute myeloid leukemia defines cell cycle and immunophenotypic properties that correlate with known responses to therapy." Cancer discovery (2015): CD-15. Gaudillière, Brice, et al. "Implementing mass cytometry at the bedside to study the immunological basis of human diseases: distinctive immune features in patients with a history of term or preterm birth." Cytometry Part A 87.9 (2015): 817-829. Gaudilliere, Brice, et al. "Coordinated surgical immune signatures contain correlates of clinical recovery." Science translational medicine 6.255 (2014): 255ra131. Behbehani, Gregory K., et al. "Single-cell mass cytometry adapted to measurements of the cell cycle." Cytometry Part A 81.7 (2012): 552-566.

1 liter bottle of Proteomic Stabilizer PROT1. Sufficient to stabilize over 700 milliliters of whole blood when used as recommended. Also includes buffer used after thawing samples stored frozen in Stabilizer: THAWLYSE1 "Thaw-Lyse, 1000X Concentrate". Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351691 Protocol for banking samples with PROT1 Protocol for thawing samples banked with PROT1 Published antibody panels used to analyze samples banked in PROT1   Published Protocols and Methods Papers using PROT1 Smets, Tina, et al. "Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)." Multiple Myeloma. Humana Press, New York, NY, 2018. 47-54. Behbehani, Gregory K. "Cell Cycle Analysis by Mass Cytometry." Cellular Quiescence. Humana Press, New York, NY, 2018. 105-124. Fernandez, Rosemary, and Holden Maecker. "Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry." Bio-protocol 5.11 (2015).   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Geanon, Daniel, et al. "A Streamlined CyTOF Workflow To Facilitate Standardized Multi-Site Immune Profiling of COVID-19 Patients." medRxiv 2020.06.26.20141341 (2020). Rahil, Zainab, et al. "Landscape of coordinated immune responses to H1N1 challenge in humans." Journal of Clinical Investigation, Vol. 130 (2020). Brandt, Raphael, et al. "Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium." Nature Communications, Vol. 10, Article 2919 (2019). Tsai, Amy, et al. "A year-long immune profile of the systemic response in acute stroke survivors." Brain, Vol. 142, Iss. 4 (2019). Chen, Luxi, et al. "CD56 Expression Marks Human Group 2 Innate Lymphoid Cell Divergence from a Shared NK Cell and Group 3 Innate Lymphoid Cell Developmental Pathway." Immunity (2018).  Ajami, Bahareh, et al. "Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models." Nature Neuroscience 21.4 (2018): 541. Spitzer, Matthew H., et al. "Systemic immunity is required for effective cancer immunotherapy." Cell 168.3 (2017): 487-502. Glassberg, Jeffrey, et al. "Application of phospho-CyTOF to characterize immune activation in patients with sickle cell disease in an ex vivo model of thrombosis." Journal of immunological methods 453 (2018): 11-19. Blazkova, Jana, et al. "Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy." The Journal of Immunology (2017): 1601855. Schulz, Axel Ronald, et al. "Silver nanoparticles for the detection of cell surface antigens in mass cytometry." Cytometry Part A 91.1 (2017): 25-33. Ciarlo, Eleonora, et al. "Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo." Scientific reports 6 (2016): 37944. Fragiadakis, Gabriela K., et al. "Patient-specific immune states before surgery are strong correlates of surgical recovery." Anesthesiology: The Journal of the American Society of Anesthesiologists 123.6 (2015): 1241-1255. Spitzer, Matthew H., et al. "An interactive reference framework for modeling a dynamic immune system." Science 349.6244 (2015): 1259425. Behbehani, Gregory K., et al. "Mass cytometric functional profiling of acute myeloid leukemia defines cell cycle and immunophenotypic properties that correlate with known responses to therapy." Cancer discovery (2015): CD-15. Gaudillière, Brice, et al. "Implementing mass cytometry at the bedside to study the immunological basis of human diseases: distinctive immune features in patients with a history of term or preterm birth." Cytometry Part A 87.9 (2015): 817-829. Gaudilliere, Brice, et al. "Coordinated surgical immune signatures contain correlates of clinical recovery." Science translational medicine 6.255 (2014): 255ra131. Behbehani, Gregory K., et al. "Single-cell mass cytometry adapted to measurements of the cell cycle." Cytometry Part A 81.7 (2012): 552-566.

Bottle of Proteomic Stabilizer. Volume sufficient for stabilizing one hundred 0.5ml samples of whole blood. Also includes buffer used after thawing samples stored frozen in Stabilizer: THAWLYSE1 "Thaw-Lyse, 1000X Concentrate".  Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351689 Protocol for banking samples with PROT1 Protocol for thawing samples banked with PROT1 Published antibody panels used to analyze samples banked in PROT1 Published Protocols and Methods Papers using PROT1 Smets, Tina, et al. "Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)." Multiple Myeloma. Humana Press, New York, NY, 2018. 47-54. Behbehani, Gregory K. "Cell Cycle Analysis by Mass Cytometry." Cellular Quiescence. Humana Press, New York, NY, 2018. 105-124. Fernandez, Rosemary, and Holden Maecker. "Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry." Bio-protocol 5.11 (2015).   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Geanon, Daniel, et al. "A Streamlined CyTOF Workflow To Facilitate Standardized Multi-Site Immune Profiling of COVID-19 Patients." medRxiv 2020.06.26.20141341 (2020). Rahil, Zainab, et al. "Landscape of coordinated immune responses to H1N1 challenge in humans." Journal of Clinical Investigation, Vol. 130 (2020). Brandt, Raphael, et al. "Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium." Nature Communications, Vol. 10, Article 2919 (2019). Tsai, Amy, et al. "A year-long immune profile of the systemic response in acute stroke survivors." Brain, Vol. 142, Iss. 4 (2019). Chen, Luxi, et al. "CD56 Expression Marks Human Group 2 Innate Lymphoid Cell Divergence from a Shared NK Cell and Group 3 Innate Lymphoid Cell Developmental Pathway." Immunity (2018).  Ajami, Bahareh, et al. "Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models." Nature Neuroscience 21.4 (2018): 541. Spitzer, Matthew H., et al. "Systemic immunity is required for effective cancer immunotherapy." Cell 168.3 (2017): 487-502. Glassberg, Jeffrey, et al. "Application of phospho-CyTOF to characterize immune activation in patients with sickle cell disease in an ex vivo model of thrombosis." Journal of immunological methods 453 (2018): 11-19. Blazkova, Jana, et al. "Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy." The Journal of Immunology (2017): 1601855. Schulz, Axel Ronald, et al. "Silver nanoparticles for the detection of cell surface antigens in mass cytometry." Cytometry Part A 91.1 (2017): 25-33. Ciarlo, Eleonora, et al. "Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo." Scientific reports 6 (2016): 37944. Fragiadakis, Gabriela K., et al. "Patient-specific immune states before surgery are strong correlates of surgical recovery." Anesthesiology: The Journal of the American Society of Anesthesiologists 123.6 (2015): 1241-1255. Spitzer, Matthew H., et al. "An interactive reference framework for modeling a dynamic immune system." Science 349.6244 (2015): 1259425. Behbehani, Gregory K., et al. "Mass cytometric functional profiling of acute myeloid leukemia defines cell cycle and immunophenotypic properties that correlate with known responses to therapy." Cancer discovery (2015): CD-15. Gaudillière, Brice, et al. "Implementing mass cytometry at the bedside to study the immunological basis of human diseases: distinctive immune features in patients with a history of term or preterm birth." Cytometry Part A 87.9 (2015): 817-829. Gaudilliere, Brice, et al. "Coordinated surgical immune signatures contain correlates of clinical recovery." Science translational medicine 6.255 (2014): 255ra131. Behbehani, Gregory K., et al. "Single-cell mass cytometry adapted to measurements of the cell cycle." Cytometry Part A 81.7 (2012): 552-566.

250ml bottle of Stable-Lyse V2 buffer. This buffer is used in conjunction with Stable-Store V2 buffer to bank whole blood samples for temporary storage at 4 degrees Celsius and long-term banking at -80 degrees Celsius. Use 350 microliters per 250 microliters of whole blood. Not to be used with standard Smart Tubes or with the PROT1 Proteomic Stabilizer.  Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351694 Protocols for using Stable-Lyse and Stable-Store Reagent System   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Lee, Amy et al. "Dynamic molecular changes during the first week of human life follow a robust developmental trajectory" Nature Communications (2019).

250ml bottle of Stable-Store V2 buffer. This buffer is used in conjunction with Stable-Lyse V2 buffer to bank whole blood samples for temporary storage at 4 degrees Celsius and long-term banking at -80 degrees Celsius. Protocols for using Stable-Lyse and Stable-Store Reagent System   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Lee, Amy et al. "Dynamic molecular changes during the first week of human life follow a robust developmental trajectory" Nature Communications (2019).

1000ml bottle of Stable-Store V2 buffer. This buffer is used in conjunction with Stable-Lyse V2 buffer to bank whole blood samples for temporary storage at 4 degrees Celsius and long-term banking at -80 degrees Celsius. Use 1000 microliters per 250 microliters of whole blood. Not to be used with standard Smart Tubes or with the PROT1 Proteomic Stabilizer.  Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351693 Protocols for using Stable-Lyse and Stable-Store Reagent System   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used this buffer to bank their samples Lee, Amy et al. "Dynamic molecular changes during the first week of human life follow a robust developmental trajectory" Nature Communications (2019).

Standard Smart Tube, case of one hundred. Optimized to work with 1 milliliter of whole blood. Includes THAWLYSE1 "Thaw-Lyse, 1000X Concentrate" the buffer used after thawing samples stored frozen in Smart Tubes. Also includes SERT1 "a bag of one hundred (100) Processing Inserts" used to process the thawed sample. Inquire about Smart Tubes optimized to work with other sample volumes as well as Smart Tubes pre-loaded with biologics or drugs of interest.  Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351690 Protocol for incubating and freezing samples in Smart Tubes without using a Base Station Protocol for thawing samples frozen in Smart Tubes Published antibody panels used to analyze samples banked in Smart Tubes Published Protocols and Methods Papers using Smart Tubes MTS1P Smets, Tina, et al. "Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)." Multiple Myeloma. Humana Press, New York, NY, 2018. 47-54.   See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples. Publications that used Smart Tubes to bank their samples include: Adams, Homer, et al. "High‐Parameter Mass Cytometry Evaluation of Relapsed/Refractory Multiple Myeloma Patients Treated with Daratumumab Demonstrates Immune Modulation as a Novel Mechanism of Action." Cytometry, 95: 279-289 (2019). Aghaeepour, Nima, et al. "An immune clock of human pregnancy." Science immunology 2.15 (2017): eaan2946. Aghaeepour, Nima, et al. "Deep Immune Profiling of an Arginine-Enriched Nutritional Intervention in Patients Undergoing Surgery." The Journal of Immunology (2017): ji1700421. Furman, David, et al. "Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states." Nature medicine 23.2 (2017): 174. Gaudillière, Brice, et al. "Clinical recovery from surgery correlates with single-cell immune signatures." Science translational medicine 6.255 (2014): 255ra131.

60 milliliter bottle of Thaw-Lyse Buffer, 1000X Concentrate. Thaw-Lyse Buffer is used after thawing samples stored frozen in PROT1 or Smart Tubes. Thaw-Lyse buffer lyses erythrocytes in samples previously frozen in PROT1 or Smart Tubes. This buffer is supplied as a 1000X concentrate.  Fisher Scientific Part Number: 501351696