NR-49169_R05T,Rousettusaegyptiacus(埃及果蝠）永生化胎儿细胞系(细胞库）
产品图片
货号/SKU
NR-49169
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-49169?? R05T, Rousettus aegyptiacus (Egyptian fruit bat) Immortalized Fetal Cell Line(Cell Banks)|Rousettus aegyptiacus|R05T, Rousettus aegyptiacus (Egyptian fruit bat) Immortalized Fetal Cell Line|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|I JordanAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: R05T, Rousettus aegyptiacus (Egyptian fruit bat), Immortalized Fetal Cell Line, NR-49169."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The R05T cell line was generated from cells isolated from the head of an Egyptian fruit bat (Rousettus aegyptiacus) fetus. Cultured primary cells were immortalized by transfection with an adenovirus 5 E1A/E1B expression plasmid. (R. aegyptiacus) has been identified as a natural reservoir for marburgviruses. However, experimental inoculation studies indicated very limited susceptibility to ebolavirus infection, suggesting that this species is an unlikely source for ebolaviruses in nature.
NR-49169 deposited at BEI Resources was confirmed to be of bat origin and remained free of detectable cross-contamination with cells of 12 other mammalian species, even after ten passages of the original seed cells received from the contributor.
Each vial contains approximately 1 mL of cell culture suspension frozen in DMEM:F12 medium (65%), fetal bovine serum (25%), and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
The R05T cell line was generated from cells isolated from the head of an Egyptian fruit bat (Rousettus aegyptiacus) fetus. Cultured primary cells were immortalized by transfection with an adenovirus 5 E1A/E1B expression plasmid. (R. aegyptiacus) has been identified as a natural reservoir for marburgviruses. However, experimental inoculation studies indicated very limited susceptibility to ebolavirus infection, suggesting that this species is an unlikely source for ebolaviruses in nature.
NR-49169 deposited at BEI Resources was confirmed to be of bat origin and remained free of detectable cross-contamination with cells of 12 other mammalian species, even after ten passages of the original seed cells received from the contributor.
Each vial contains approximately 1 mL of cell culture suspension frozen in DMEM:F12 medium (65%), fetal bovine serum (25%), and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID进行批准. R05T细胞系从埃及果棒的头部隔离的细胞生成（ roupetus aegyptiacus ）胎儿.通过用腺病毒5e1a / E1b表达质粒转染培养的原发性细胞. （ r.Aegyptiacus ）已被确定为Marburgviruses的天然水库.然而，实验性接种研究表明对埃博拉病毒感染的易感性非常有限，这表明该物种是艾伯吞噬物质中的一个不太可能的来源.甚至在从贡献者那里收到的原始种子细胞的10个通道之后具有12种哺乳动物物种的细胞的可检测的交叉污染. 每个小瓶在DMEM中含有大约1毫升细胞培养悬浮液：F12培养基（65 ％），胎儿牛血清（25％），和DMSO（10％）冷冻保存.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
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除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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