NR-566_单克隆抗痘苗病毒(WR)L1R蛋白,残基1至185(类似于VMC-35）,(体外生产)(单克隆抗体）
产品图片
货号/SKU
NR-566
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-566?? Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-35), (produced in vitro)(Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-35), (produced in vitro)|-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-35), (produced in vitro), NR-566."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-566 was purified from the same hybridoma as VMC-35. The specificity of VMC-35 was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. VMC-35 does not neutralize the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-566 was purified from the same hybridoma as VMC-35. The specificity of VMC-35 was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. VMC-35 does not neutralize the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到NIAID批准. L1R蛋白的重组形式的小鼠单克隆抗体[L1R（185t）;使用硫酸铵沉淀和尺寸排阻色谱法从小鼠B细胞杂交瘤中纯化西方储备（WR）疫苗病毒的基韦储备（WR）菌株的残基1至185.通过具有免疫BALB / C脾细胞的SP2 / 0骨髓瘤细胞的融合产生小鼠B细胞杂交瘤.将NR-566与与VMC-35相同的杂交瘤纯化. VMC-35的特异性通过ELISA的反应性决定通过ELISA对L1R（185t）确定，并通过在还原和非还原条件下通过Western印迹分析证实. VMC-35使用IMV斑块还原测定不会中和BS-C-1细胞中痘苗病毒的细胞内成熟病毒（IMV）形式的感染性. 每个小瓶含有约1.0ml纯化的单克隆抗体在50毫米硼酸盐（pH 8.0 +/- 0.2）中含有0.1米氯化钠，无防腐剂.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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