NR-53260抗SARS冠状病毒人单克隆抗体CR3022质粒组(质粒/载体）
产品图片
货号/SKU
NR-53260
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-53260?? Plasmid Set for Anti-SARS Coronavirus Human Monoclonal Antibody CR3022(Plasmid/Vectors)|SARS Coronavirus|Plasmid Set for Anti-SARS Coronavirus Human Monoclonal Antibody CR3022| -20°C or colder|F Krammer, V Chromikova, G Asthagiri ArunkumarAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Plasmid Set for Anti-SARS Coronavirus Human Monoclonal Antibody CR3022, NR-53260.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The vectors for human anti-severe acute respiratory syndrome coronavirus (SARS-CoV) immunoglobulin heavy and light chain variable regions (CR3022 vH and vL, GenBank: DQ168569 and DQ168570, respectively) were designed for transient co-transfection in mammalian cells for expression of recombinant SARS-CoV neutralizing antibody CR3022. CR3022 targets a highly conserved epitope that enables cross-reactive binding in both SARS-CoV and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2).
NR-53260 consists of the two plasmids listed in Table 1. Descriptions of each component are included below.
Table 1: CR3022 Plasmid Set
The CR3022 vH and vL sequences were subcloned into mammalian expression vectors (pFUSEss-CHIg-hG1 and pFUSEss-CLIg-hk, respectively) fused to the N-terminal interleukin 2 (IL2) signal sequence and the C-terminal constant regions of human IgG1 (hIgG1) heavy or human Ig kappa (hIgκ) light chain. NR-52399 contains the Streptoalloteichus hindustanus bleomycin (Sh ble) gene to provide transformant selection through Zeocin? resistance in Escherichia coli (E. coli) and mammalian cells. NR-52400 contains the Bacillus cereus blasticidin-S deaminase (bsr) gene to provide transformant selection through blasticidin resistance in E. coli and mammalian cells. The resulting sizes of plasmids are approximately 4830 and 4190 base pairs, respectively. The complete plasmid sequences and maps are provided on the BEI Resources webpage. The plasmids were produced in E. coli and extracted.
Note: NR-52399 and NR-52400 must be co-transfected to express the CR3022 monoclonal antibody.
Each kit contains one vial of each plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. Each vial should be centrifuged prior to opening. Note: The contents of each vial should be used to replicate each plasmid in E. coli prior to mammalian expression.
The vectors for human anti-severe acute respiratory syndrome coronavirus (SARS-CoV) immunoglobulin heavy and light chain variable regions (CR3022 vH and vL, GenBank: DQ168569 and DQ168570, respectively) were designed for transient co-transfection in mammalian cells for expression of recombinant SARS-CoV neutralizing antibody CR3022. CR3022 targets a highly conserved epitope that enables cross-reactive binding in both SARS-CoV and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2).
NR-53260 consists of the two plasmids listed in Table 1. Descriptions of each component are included below.
Table 1: CR3022 Plasmid Set
Plasmid Type |
Insert |
Catalog Number |
|---|---|---|
The CR3022 vH and vL sequences were subcloned into mammalian expression vectors (pFUSEss-CHIg-hG1 and pFUSEss-CLIg-hk, respectively) fused to the N-terminal interleukin 2 (IL2) signal sequence and the C-terminal constant regions of human IgG1 (hIgG1) heavy or human Ig kappa (hIgκ) light chain. NR-52399 contains the Streptoalloteichus hindustanus bleomycin (Sh ble) gene to provide transformant selection through Zeocin? resistance in Escherichia coli (E. coli) and mammalian cells. NR-52400 contains the Bacillus cereus blasticidin-S deaminase (bsr) gene to provide transformant selection through blasticidin resistance in E. coli and mammalian cells. The resulting sizes of plasmids are approximately 4830 and 4190 base pairs, respectively. The complete plasmid sequences and maps are provided on the BEI Resources webpage. The plasmids were produced in E. coli and extracted.
Note: NR-52399 and NR-52400 must be co-transfected to express the CR3022 monoclonal antibody.
Each kit contains one vial of each plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. Each vial should be centrifuged prior to opening. Note: The contents of each vial should be used to replicate each plasmid in E. coli prior to mammalian expression.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
人抗重型急性呼吸综合征冠状病毒（SARS-COV）免疫球蛋白重和轻链可变区的载体（CR3022 VH和VL，Genbank： dq168569 和 DQ168570 ，分别为瞬态共转染在哺乳动物细胞中表达重组SARS-COV中和抗体CR3022. CR3022针对一个高度保守的表位，使SARS-COV和严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2）中的交叉反应性.
NR-53260由表1中列出的两种质粒组成.每个组分的描述包括下面.
表1：Cr3022质粒套装
质粒类型
插入
bei资源目录号
人IgG1
CR3022重链
nr-52399
人IGκ
CR3022轻链
nr-52400
将CR3022 VH和VL序列亚克隆到哺乳动物表达载体（ pfusess-chig-hg1中和 pfusess-clig-hk ，分别融合对于N-末端白细胞介素2（IL2）信号序列和人IgG1（HIGG1）重或人Ig Kappa（Higκ）轻链的C末端常数区域. NR-52399含有链霉素Hindustanus Bleomycin （Sh ble）基因，通过在大肠杆菌（大肠杆菌）中通过Zeocin TM抗性提供转化体选择和哺乳动物细胞. NR-52400.
含有芽孢杆菌Cereus Blasticidin-s deaminase （BSR）基因，通过在 e中通过Blasticidin抗性提供转化体选择. COLI 和哺乳动物细胞.得到的质粒的尺寸分别是约4830和4190个碱基对.完全质粒序列和地图是在BEI资源网页上提供的.在 e中制备质粒. Coli 并提取.
注意：必须共转染NR-52399和NR-52400以表达CR3022单克隆抗体.
每个试剂盒在10mM Tris-HCl，1mM EDTA，pH8.0中含有一个小瓶.每个小瓶应该是
在打开之前离心. 注意：每个小瓶的内容应该用于在 e中复制每种质粒. Coli 在哺乳动物表达之前.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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