NR-50146_来自波旁病毒的基因组 RNA

NR-50146?? Genomic RNA from Bourbon Virus, Original (Nucleic Acids)|Bourbon virus| Genomic RNA from Bourbon Virus, Original |-60°C or colder|BJ RussellAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic RNA from Bourbon Virus, Original, NR-50146."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic RNA was isolated from a preparation of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero E6; ATCC ^? CRL-1586?) infected with Bourbon virus (BRBV), Original. BRBV, Original was isolated from a human with fever, thrombocytopenia, and a recent history of tick exposure in Bourbon County, Kansas, in June 2014.  NR-50146 has been qualified for PCR applications by amplification of an approximately 750 nucleotide sequence. Each vial contains 100 ?L of viral genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.