NR-29402_肠沙门氏菌亚种.enterica,14028s(SerovarTyphimurium)Single-GeneDeletionMutantLibrary,Plate007/008_Kan(MutantBacteria)
产品图片
货号/SKU
NR-29402
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-29402??Salmonella enterica subsp. enterica, 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 007/008_Kan(Mutant Bacteria)|Salmonella enterica subsp. enterica|14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 007/008_Kan|-80°C or colder|H Andrews-Polymenis, M McClellandAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 007/008_Kan, NR-29402."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The Salmonella enterica (S. enterica) subsp. enterica, strain 14028s (serovar Typhimurium) targeted single-gene deletion (SGD) mutant library contains a total of 3,773 individual genes deleted simultaneously across two collections of mutants differentiated by kanamycin or chloramphenicol resistance. The kanamycin-resistant mutant collection contains 3,517 mutants distributed among eleven 96-well plates. In these mutants, a single gene is replaced by a cassette conferring the kanamycin resistance gene, and includes 9 double mutants that contain both kanamycin and chloramphenicol cassettes. Deletions were confirmed by the depositor. The parent strain S. enterica subsp. enterica, strain 14028s is available from BEI Resources as NR-12154.
Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid pCLF4 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Luria Bertani (LB) broth containing 60 ?g/mL kanamycin supplemented with 10% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The Salmonella enterica (S. enterica) subsp. enterica, strain 14028s (serovar Typhimurium) targeted single-gene deletion (SGD) mutant library contains a total of 3,773 individual genes deleted simultaneously across two collections of mutants differentiated by kanamycin or chloramphenicol resistance. The kanamycin-resistant mutant collection contains 3,517 mutants distributed among eleven 96-well plates. In these mutants, a single gene is replaced by a cassette conferring the kanamycin resistance gene, and includes 9 double mutants that contain both kanamycin and chloramphenicol cassettes. Deletions were confirmed by the depositor. The parent strain S. enterica subsp. enterica, strain 14028s is available from BEI Resources as NR-12154.
Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid pCLF4 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Luria Bertani (LB) broth containing 60 ?g/mL kanamycin supplemented with 10% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
沙门氏菌肠（S. enterica）子块. 肠杆菌，菌株14028s（Serovar Typhimurium）靶向单基因缺失（SGD）突变文库含有总共3,773个单独的基因，横跨由卡那霉素或氯霉素抗性分化的两种突变体.卡那霉素抗性突变体收集含有3,517个突变体，其中96孔板中分布.在这些突变体中，单个基因被赋予卡那霉素抗性基因的盒子代替，并且包括含有卡那霉素和氯霉素盒的9个双突变体.赔偿者确认删除.父株 s. enterica 子公司. entenica ，应变14028S可从BEI资源作为NR-12154获得.
靶向基因旨在通过旨在保留每种缺失基因的第一和最后30个碱基的引物缺失.基因更换遵循改性的λ-红色技术，其中添加的T7 RNA聚合酶启动子位于质粒 pclf4 从每种突变体的下游产生来自沙门氏菌的基因组的基因特异性转录物.
96孔板的每个接种井在含有60μg/ ml卡那霉素的Luria Bertani（LB）肉汤中含有大约50μl培养物，其补充有10％甘油.
96孔格式的生产增加了相邻井之间的交叉污染的风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei资源不确认或验证贡献者提供的个体突变体.
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