来自大肠杆菌的重组(毒素），NR-1_Vaccinia virus

NR-51681?? Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli(Toxins)|Escherichia coli|Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli| 2°C to 8°C|JP BitounAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli, NR-51681.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-51681 is a recombinant toxoid of enterotoxigenic Escherichia coli (E. coli) (ETEC) heat-labile toxin (LT) with a double mutation (R192G/L211A; based on the recombinant sequence). The recombinant double mutant, dmLT or LT(R192G/L211A), has reduced proteolytic activation and enterotoxicity while maintaining adjuvanticity equal to native LT. NR-51681 was expressed in E. coli and purified by immobilized galactose chromatography. The theoretical molecular weight is approximately 86 kilodaltons. The amino acid sequence of both subunit A and subunit B of heat-labile toxin have been determined (GenPept: P06717 and P32890, respectively). The crystal structure of heat-labile toxin complexed with lactose has been determined to 2.3 ? (PDB: 1LTT). NR-51681 is functional in SDS-PAGE, cAMP assays in human colorectal carcinoma (T84) cells, western blot and oral adjuvanticity (ELISA) assays. The novel dmLT adjuvant promotes dose sparing, mucosal immunity and longevity of antibody responses to the inactivated polio vaccine in a murine model. Each vial of NR-51681 contains approximately 1 mg of purified recombinant dmLT adjuvant, which was lyophilized from TEAN (Tris, EDTA, sodium azide and sodium chloride) buffer.

NR-10357?? Perfringolysin O from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli(Toxins)|Clostridium perfringens|Perfringolysin O from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli|-80°C or colder|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Perfringolysin O from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-10357."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Clostridium perfringens (C. perfringens) perfringolysin O is a thiol-activated cytolysin that binds to membrane cholesterol and forms oligomeric pores, causing membrane damage.NR-10357 is a recombinant form of perfringolysin O from C. perfringens. The recombinant protein was cloned from ATCC ? 13124? and contains an N-terminal histidine tag. NR-10357 was expressed in Escherichia coli and purified using standard chromatographic methods. NR-10357 has been qualified for western blots and cytolytic assays.Each vial of NR-10357 contains approximately 25 ?g of perfringolysin O in 50 mM HEPES (pH 8.0) containing 10% glycerol, 150 mM NaCl and 5 mM DTT.