NR-51681肠毒素大肠杆菌大肠杆菌双突变体热不稳定毒素(DMLT）,佐剂活性,来自大肠杆菌的重组(毒素）
产品图片
货号/SKU
NR-51681
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-51681?? Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli(Toxins)|Escherichia coli|Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli| 2°C
to 8°C|JP BitounAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Double Mutant Heat-Labile Toxoid (dmLT), Adjuvant-Active, Recombinant from Escherichia coli, NR-51681.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-51681 is a recombinant toxoid of enterotoxigenic Escherichia coli (E. coli) (ETEC) heat-labile toxin (LT) with a double mutation (R192G/L211A; based on the recombinant sequence). The recombinant double mutant, dmLT or LT(R192G/L211A), has reduced proteolytic activation and enterotoxicity while maintaining adjuvanticity equal to native LT. NR-51681 was expressed in E. coli and purified by immobilized galactose chromatography. The theoretical molecular weight is approximately 86 kilodaltons.
The amino acid sequence of both subunit A and subunit B of heat-labile toxin have been determined (GenPept: P06717 and P32890, respectively). The crystal structure of heat-labile toxin complexed with lactose has been determined to 2.3 ? (PDB: 1LTT).
NR-51681 is functional in SDS-PAGE, cAMP assays in human colorectal carcinoma (T84) cells, western blot and oral adjuvanticity (ELISA) assays. The novel dmLT adjuvant promotes dose sparing, mucosal immunity and longevity of antibody responses to the inactivated polio vaccine in a murine model.
Each vial of NR-51681 contains approximately 1 mg of purified recombinant dmLT adjuvant, which was lyophilized from TEAN (Tris, EDTA, sodium azide and sodium chloride) buffer.
NR-51681 is a recombinant toxoid of enterotoxigenic Escherichia coli (E. coli) (ETEC) heat-labile toxin (LT) with a double mutation (R192G/L211A; based on the recombinant sequence). The recombinant double mutant, dmLT or LT(R192G/L211A), has reduced proteolytic activation and enterotoxicity while maintaining adjuvanticity equal to native LT. NR-51681 was expressed in E. coli and purified by immobilized galactose chromatography. The theoretical molecular weight is approximately 86 kilodaltons.
The amino acid sequence of both subunit A and subunit B of heat-labile toxin have been determined (GenPept: P06717 and P32890, respectively). The crystal structure of heat-labile toxin complexed with lactose has been determined to 2.3 ? (PDB: 1LTT).
NR-51681 is functional in SDS-PAGE, cAMP assays in human colorectal carcinoma (T84) cells, western blot and oral adjuvanticity (ELISA) assays. The novel dmLT adjuvant promotes dose sparing, mucosal immunity and longevity of antibody responses to the inactivated polio vaccine in a murine model.
Each vial of NR-51681 contains approximately 1 mg of purified recombinant dmLT adjuvant, which was lyophilized from TEAN (Tris, EDTA, sodium azide and sodium chloride) buffer.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-51681是具有双突变（R192G / L211A;基于重组序列的肠毒素的肠毒素大肠杆菌（大肠杆菌）的重组毒素（大肠杆菌）的重组毒素（大肠杆菌）的热 - 不稳定毒素（LT）.重组双突变体DMLT或LT（R192G / L211A）降低了蛋白水解活化和肠毒性，同时保持等于天然LT的佐性. NR-51681在 e中表达. Coli 通过固定的半乳糖色谱法纯化.理论分子量约为86千杆翁间.
已经确定（Genpept：
p32890 ，
分别）.用乳糖复合的热不稳定毒素的晶体结构已被确定为2.3?（pdb： 1ltt ）.
NR-51681是在SDS-PAGE中的功能性，人结肠直肠癌（T84）细胞，蛋白质印迹和口服辅助（ELISA）测定中的CAMP测定.新型DMLT佐剂促进了对小鼠模型中的灭活脊髓灰质炎疫苗的抗体反应的剂量备件，粘膜免疫和寿命.
NR-51681的每个小瓶含有约1mg纯化的重组DMLT佐剂，其从Tean（Tris，EDTA，叠氮化钠和氯化钠）缓冲液中冻干.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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