NR-15783结核分枝杆菌、CDC1551、转座子突变体敲除池11(突变细菌)
产品图片
货号/SKU
NR-15783
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15783??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 11(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 11|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 11, NR-15783."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 11 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 11 are available individually (see Pool 11 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 11 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 11 are available individually (see Pool 11 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
PROBABLE NUCLEOTIDE-SUGAR EPIMERASE EPIA | MT1562 | Rv1512 | NA |
PROBABLE POLYKETIDE BETA-KETOACYL SYNTHASE PKS4 | MT1218 | Rv1181 | NA |
PROBABLE PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA | MT0145 | Rv0137c | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT1820 | Rv1769 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT1622.1 | Rv1572c | NA |
ARABINOSYL TRANSFERASE | MT3900 | Rv3793 | NA |
PPE FAMILY PROTEIN | MT0469 | Rv0453 | NA |
PROBABLE AMIDASE AMIB2 (AMINOHYDROLASE) | MT1301 | Rv1263 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2273 | Rv2216 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2160 | Rv2100 | NA |
PROBABLE TETRONASIN-TRANSPORT INTEGRAL MEMBRANE PROTEIN ABC TRANSPORTER | MT01255 | Rv1217c | NA |
HYPOTHETICAL PROTEIN | MT3511 | Rv3403c | NA |
MCE-FAMILY PROTEIN MCE1B | MT0179 | Rv0170 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT3590 | Rv3486 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT3753 | Rv3651 | NA |
POSSIBLE MAGNESIUM CHELATASE | MT2916 | Rv2850c | NA |
POSSIBLE Mg 2+ TRANSPORT P-TYPE ATPASE C MGTC | MT1859 | Rv1811 | NA |
PPE FAMILY PROTEIN | MT1234 | Rv1196 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT3721 | Rv3619c | NR-15136 |
POSSIBLE 8-AMINO-7-OXONONANOATE SYNTHASE BIOF2 | MT0037 | Rv0032 | NA |
1Rv*- In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA- Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到北欧批准. 目标(结核病形成动物研究和基因评估任务性),以使使用定义的方案和测试定义的 m的定义突变体来实现多种动物物种中的人结核病的建模.结核病.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突变池11被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池11中的一些转座子突变体可单独使用(参见下面的池11个组件). 每个小瓶在含有100μg/ ml环己酰胺的OADC富集中含有约1ml的细菌培养物和20μg/ ml kanamycin. 组件: 转座子敲除突变体的描述 菌株 cdc1551基因 菌株H37RV 基因 1 bei资源 产品编号 2 可能核苷酸 - 糖映像酶EPIA Mt1562 RV1512 Na 可能聚酮β-酮酮合成酶PKS4 Mt1218 RV1181 Na 可能肽甲硫氨酸亚硫氧化物还原酶MSRA Mt0145 RV0137C Na 保守假设蛋白 mt1820 rv1769 na 保守假设蛋白 mt1622.1 Rv1572c na 阿拉伯糖基转移酶 mt3900 rv3793 na ppe家族蛋白 mt0469 rv0453 na 可能酰胺酶Amib2(氨基水解酶) MT1301 RV1263 Na 保守假设蛋白 MT2273 RV2216 Na 保守假设蛋白 Mt2160 RV2100 Na 可能左旋松素 - 传输整体膜蛋白ABC转运蛋白ABC转运蛋白 RV1217C Na 假设蛋白 mt3511 Rv3403c na MCE-Family蛋白MCE1B Mt0179 RV0170 Na 保守假设蛋白 mt3590 rv3486 na 保守假设蛋白 mt3753 RV3651 Na 可能的镁切酶 MT2916 RV2850C na 可能的mg 2+ 运输p型ATPase C mgtc mt1859 RV1811 na ppe家族蛋白 mt1234 RV1196 Na 保守假设蛋白 mt3721 rv3619c nr-15136 可能8-氨基-7-氧代壬酸酯合酶BioF2 Mt0037 RV0032 Na 1 rv * - 在某些情况下,没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
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