NR-15782结核分枝杆菌、CDC1551、转座子突变体敲除池10(突变细菌)
产品图片
货号/SKU
NR-15782
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15782??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 10(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 10|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 10, NR-15782."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 10 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 10 are available individually (see Pool 10 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 10 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 10 are available individually (see Pool 10 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
PROBABLE CONSERVED TRANSMEMBRANE PROTEIN | MT3826 | Rv3723 | NR-15707 |
PROBABLE OXIDASE | MT0869 | Rv0846c | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2707 | Rv2631 | NR-15084 |
SECRETED L-ALANINE DEHYDROGENASE | MT2850 | Rv2780 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2634 | Rv2557 | NA |
PROBABLE THIOL PEROXIDASE | MT1982 | Rv1932 | NR-15710 |
CONSERVED HYPOTHETICAL PROTEIN. POSSIBLE S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE | MT0154 | Rv0146 | NR-15709 |
CONSERVED HYPOTHETICAL PROTEIN | MT2082 | Rv2024c | NR-15728 |
CONSERVED HYPOTHETICAL PROTEIN | MT2061 | Rv2005c | NR-15085 |
HYPOTHETICAL PROTEIN | MT1965 | Rv1914c | NA |
PPE FAMILY PROTEIN | MT2683 | Rv2608 | NR-17556 |
PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN | MT3335 | Rv3238c | NA |
HYPOTHETICAL PROTEIN | MT3954 | Rv* | NR-17558 |
HYPOTHETICAL TRANSCRIPTIONAL REGULATORY PROTEIN | MT3179 | Rv3095 | NR-18223 |
POSSIBLE PHOSPHOGLYCERATE MUTASE | MT3310 | Rv3214 | NR-17561 |
CONSERVED HYPOTHETICAL PROTEIN | MT0306 | Rv0293c | NR-17562 |
PROBABLE PHOSPHATE-TRANSPORT SYSTEM TRANSCRIPTIONAL REGULATORY PROTEIN | MT3400 | Rv3301c | NR-17560 |
MEMBRANE-ANCHORED ADENYLYL CYCLASE | MT1661 | Rv1625c | NR-17563 |
PROBABLE ACYL-CoA DEHYDROGENASE | MT3904 | Rv3797 | NR-17582 |
PROBABLE OXIDOREDUCTASE | MT1298 | Rv1260 | NR-17564 |
1Rv* - In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA - Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. target (结核病动物研究和基因评估任务)形成为使用定义的方案和测试分枝杆菌(结核病)的定义的突变体来实现多种动物物种中的人结核病建模.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突变池10被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池10中的一些转座子突变体可单独使用(参见下面的池10个组分).每个小瓶中含有大约1毫升含有100μg/ mL环己酰亚胺的OADC富集的1ml细菌培养物和20μg/ ml kanamycin. .customtable {border:0px;单元填充:5px;细胞间距:5px;}.定制TD,TH {边框:0PX;垂直对齐:底部;}#col1 {text-align:left;宽度:55%;}#col2 {text-align:center;宽度:15%;}#col3 {text-align:center;宽度:15%;}#col4 {text-align:center;宽度:15%;} 转座子敲除突变体的描述 菌株 cdc1551 基因 菌株 基因 1 bei资源产品 2 可能保守跨膜蛋白 mt3826 RV3723 nr-15707 可能氧化酶 mt0869 RV0846C NA 保守假设蛋白 MT2707 rv2631 NR-15084 分泌的L-丙氨酸e脱氢酶 mt2850 RV2780 NA 保守假设蛋白 mt2634 RV2557 Na 可能硫醇过氧化物酶 mt1982 RV1932 NR-15710 保守假设蛋白质.可能的S-腺苷甲硫氨酸依赖性甲基转移酶 MT0154 RV0146 保守假设蛋白 mt2082 rv2024c NR-15728 保守假设蛋白 mt2061 RV2005C NR-15085 假设蛋白 mt1965 RV1914C na ppe家族蛋白 mt2683 rv2608 nr -17556 可能保守的整体膜蛋白 mt3335 RV3238C NA 假设蛋白 MT3954 RV * nr-17558 假设转录调节蛋白 mt3179 rv3095 可能的磷酸性磷酸钙蛋白酶 mt3310 RV3214 nr-17561 保守假设蛋白质 mt0306 rv0293c 可能磷酸盐 - 传输系统转录调节蛋白 mt3400 Rv3301c nr-17560 膜锚定adenylyl环形酶 MT1661 RV1625C nr-17563 可能酰基 - 辅助脱氢酶 mt3904 rv3797 NR-17582 可能氧化还原酶 MT1298 rv1260 NR-17564 1 RV * - 在某些情况下没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
厂牌介绍
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