产品描述信息
NR-15036??Mycobacterium tuberculosis, CDC1551, Transposon Mutant 1210 (MT2534, Rv2459)(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant 1210 (MT2534, Rv2459)| -60°C or colder|WR Bishai and NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant 1210 (MT2534, Rv2459), NR-15036.”|
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mycobacterium tuberculosis (M. tuberculosis), strain CDC1551 is a clinical isolate that exhibited high levels of infectivity and virulence during a tuberculosis outbreak that occurred in rural Kentucky and Tennessee from 1994 to 1996.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined
protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a
library of intragenic transposon mutants has been created and characterized. M. tuberculosis, transposon mutant 1210 was created by disruption of a probable conserved integral membrane transport protein (MT2534, Rv2459) of the wild-type strain CDC1551.
Each vial contains approximately 0.7 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment with 10% glycerol. Each tube of lot 59412007 contains a Lowenstein-Jensen (LJ) agar slant that was inoculated with 0.1 mL of bacterial culture and incubated for 2 to 8 weeks at 37°C in an aerobic atmosphere.
TARGET:
MT2534
MycoBrowser: Gene
Rv2459
ORF Description: Probable conserved integral membrane transport protein
ORF Size: 1572
POI: 404
Additional information is available at
Mycobacterium tuberculosis CDC1551 Transposon Mutant Library.
This mutant was developed by Lamichhane, et al. at the Center for Tuberculosis Research, The Johns Hopkins University School of Medicine, PubMed:
12775759.