克隆 F7-236(体外生产)(单克隆抗体），NR-1_Vaccinia virus

NR-31689?? Monoclonal Anti-Influenza A Virus Polymerase Acidic Subunit (PA), Clone F7-236 (produced in vitro)(Monoclonal Antibodies)|Influenza A virus|Monoclonal Anti-Influenza A Virus Polymerase Acidic Subunit (PA), Clone F7-236 (produced in vitro)|-20°C or colder|T TakimotoAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Influenza A Virus Polymerase Acidic Subunit (PA), Clone F7-236 (produced in vitro), NR-31689."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Antibody Class: IgG1κ^*Mouse monoclonal antibody prepared against the polymerase acidic subunit (PA) of influenza A virus was purified from clone F7-236 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0 mouse myeloma cells with splenocytes from BALB/c mice immunized by subcutaneous and intraperitoneal injection with purified RNA-dependent RNA polymerase complex from influenza virus A/chicken/Nanchang/3-120/2001 (H3N2). The trimeric polymerase complex used for immunization was prepared from Trichoplusia ni insect cells coinfected with three recombinant baculoviruses expressing the individual polymerase subunits.^*The isotype of the clone F7-236 hybridoma is reported to be IgG1. However, NR-31689 also contains a small amount of IgG2b.NR-31689 binds to both the purified polymerase complex and the PA subunit from influenza A virus A/chicken/Nanchang/3-120/2001 (H3N2) in ELISA. See Certificate of Analysis for details. The antibody is also reported to be functional in immunocytochemistry, immunofluorescence, and immunoprecipitation assays, and to react with polymerase proteins from a variety of other influenza strains.Each vial of NR-31689 contains approximately 100 ?L of purified monoclonal antibody in PBS.