Ag85A

NR-49427??Mycobacterium tuberculosis, Ag85A, Recombinant Protein Reference Standard(Proteins)|Mycobacterium tuberculosis|Ag85A, Recombinant Protein Reference Standard|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ag85A, Recombinant Protein Reference Standard, NR-49427."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-49427 is a recombinant form of the antigen 85 complex A (Ag85A) protein. The recombinant protein consists of the native protein sequence in addition to a hexa-histidine tag. The recombinant protein was expressed in Escherichia coli BL21 (DE3) pLysS cells and purified by immobilized-metal affinity chromatography. Ag85A is one of three components (Ag85A, Ag85B, Ag85C) of the secreted immunodominant 30-32 kDa Antigen 85 Complex present in the culture filtrate of Mycobacterium tuberculosis (M. tuberculosis). Each of the three proteins are involved in cell wall formation and have been linked to disease pathogenesis through their fibronectin-binding abilities. Antigen Ag85A induces strong T-cell proliferation and interferon-? in individuals infected with M. tuberculosis. Note: This protein is provided as a reference standard and should be ordered with the corresponding plasmid (pMRLB.41; NR-13292). Each vial of NR-49427 contains approximately 400 ?g of lyophilized Ag85A in 10 mM ammonium bicarbonate. Each vial of lots 64001335 and 70021268 contain 1 mg of lyophilized Ag85A in 10 mM ammonium bicarbonate. Note: NR-49427 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.