重组表达淋巴细胞脉络丛脑膜炎病毒

NR-15499?? Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Nucleoprotein(Viruses)|Lymphocytic choriomeningitis virus|Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Nucleoprotein|-60°C or colder|MJ BuchmeierAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Nucleoprotein, NR-15499."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A cDNA clone containing the entire ORF encoding the nucleoprotein (NP) from segment S of lymphocytic choriomeningitis virus (LCMV), Armstrong 53b was inserted into the pSC11 transfer vector, bringing it under the control of vaccinia virus (VACV) p7.5 early/late promoter. Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV Western Reserve strain. Recombinant virus was selected from infected cell lysates by plaquing on 143B TK- cells.LCMV is an Arenavirus (Arenaviridae, Arenavirus) which is the etiologic agent of lymphocytic choriomeningitis.Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ^? CRL-2761?) infected with recombinant vaccinia virus, rVACV-LCMV NP.Additional information and tools are available at ViPR (Virus Pathogen Resource).

NR-15497?? Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Glycoprotein Precursor(Viruses)|Lymphocytic choriomeningitis virus|Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Glycoprotein Precursor|-60°C or colder|MJ BuchmeierAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve, Recombinant Expressing Lymphocytic Choriomeningitis Virus, Armstrong 53b Glycoprotein Precursor, NR-15497."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A cDNA clone encoding the first 363 amino acids of the glycoprotein precursor (GPC) from lymphocytic choriomeningitis virus (LCMV), Armstrong 53b was inserted into the pSC11 transfer vector, bringing it under the control of vaccinia virus (VACV) p7.5 early/late promoter. This represents about 75% of the total glycoprotein, and includes all of GP1 and approximately 100 amino acids of GP2. Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV Western Reserve strain. Recombinant virus was selected from infected cell lysates by plaquing on 143B TK- cells.LCMV is an Arenavirus (Arenaviridae, Arenavirus) which is the etiologic agent of lymphocytic choriomeningitis.Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ^? CRL-2761?) infected with vaccinia virus, rVACV-LCMV GPC.Additional information and tools are available at ViPR (Virus Pathogen Resource).