产品描述信息
NR-15493?? Vaccinia Virus, Western Reserve, Recombinant Expressing Lassa Virus, Josiah Glycoprotein Precursor(Viruses)|Lassa virus|Vaccinia Virus, Western Reserve, Recombinant Expressing Lassa Virus, Josiah Glycoprotein Precursor|-60°C or colder|MJ BuchmeierAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve, Recombinant Expressing Lassa Virus, Josiah Glycoprotein Precursor, NR-15493."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A cDNA clone containing the entire ORF encoding the glycoprotein precursor (GPC) from segment S of Lassa virus (LASV) Josiah was inserted into the pRB21 transfer vector, bringing it under the control of a synthetic vaccinia virus (VACV) early/late promoter (PSYN). Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV strain vRB12.LASV is an Arenavirus (Arenaviridae, Arenavirus) which is the etiologic agent of Lassa fever.Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC
? CRL-2761?) infected with vaccinia virus, rVACV-LASV GPC.Additional information and tools are available at
ViPR (Virus Pathogen Resource).