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NR-48814?? Bat SARS-Like Coronavirus, HKU5, Recombinant, Containing the SARS Coronavirus, Urbani Spike Glycoprotein Ectodomain(Viruses)|SARS coronavirus|Bat SARS-Like Coronavirus, HKU5, Recombinant, Containing the SARS Coronavirus, Urbani Spike Glycoprotein Ectodomain|-60°C or colder|RS BaricAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bat SARS-Like Coronavirus, HKU5, Recombinant, Containing the SARS Coronavirus, Urbani Spike Glycoprotein Ectodomain, NR-48814."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Synthetic recombinant bat severe acute respiratory syndrome (SARS)-like coronavirus (CoV), HKU5, containing the spike glycoprotein ectodomain of the SARS-CoV Urbani strain (BtCoV HKU5-SE). The complete genome of bat CoV HKU5 has been sequenced (GenBank: EF065512), as has the complete genome of SARS-CoV, Urbani (GenBank: AY278741), and a mouse-adapted variant (GenBank: DQ497008). NR-48814 is a synthetic chimeric recombinant coronavirus based on a consensus bat SARS-like coronavirus (SCoV) sequence. The region of the bat SCoV genome encoding the spike (S) glycoprotein ectodomain was replaced with the corresponding region from the SARS-CoV, Urbani genome. A histidine for tyrosine substitution at position 436, previously shown to enhance replication in mice, was also included in the S gene. This virus replicates efficiently in cell culture and in young and aged mice, where the virus targets airway and alveolar epithelial cells. Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero 76, clone E6: ATCC ^? CRL-1586?) infected with BtCoV HKU5-SE. Use of this material for any purpose by commercial or for profit entities requires a license from The University of North Carolina at Chapel Hill prior to shipment. For more information on obtaining a license to use this material please contact the UNC-CH Office of Technology Development, 100 Europa Drive, Suite 430, Chapel Hill, North Carolina 27517, Phone (919) 966-3929, Email: otd@unc.edu.

NR-44009?? Bat SARS-Like Coronavirus, Recombinant, Containing the SARS Coronavirus Urbani Strain Spike Glycoprotein Receptor-Binding Domain(Viruses)|SARS coronavirus|Bat SARS-Like Coronavirus, Recombinant, Containing the SARS Coronavirus Urbani Strain Spike Glycoprotein Receptor-Binding Domain|-60°C or colder|RS BaricAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bat SARS-Like Coronavirus, Recombinant, Containing the SARS Coronavirus Urbani Strain Spike Glycoprotein Receptor-Binding Domain, NR-44009."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Synthetic recombinant bat severe acute respiratory syndrome (SARS)-like coronavirus (CoV) containing the spike glycoprotein receptor-binding domain of the SARS-CoV Urbani strain (Bat-SRBD).The complete genome of bat SARS-CoV has been sequenced (GenBank: FJ211859), as has the spike glycoprotein gene of recombinant bat-SRBD (GenBank: FJ211860).NR-44009 is a synthetic chimeric recombinant coronavirus based on a consensus bat SARS-like coronavirus (SCoV) sequence. The bat SCoV receptor-binding domain (RBD) of the spike glycoprotein (amino acids 323-505) was replaced with the SARS-CoV, Urbani RBD (amino acids 319-518), simulating a theoretical recombination event that might occur during mixed infection in vivo. Following electroporation and serial passage, progeny virions were produced with a population genome sequence identical to that of the molecular clone. The bat-SRBD virus replicates efficiently in primate and murine cells, and also replicates in mouse lungs.Each vial contains approximately 1 mL of cell lysate and supernatant from Vero E6 (C1008) cells (ATCC ^? CRL-1586?) infected with bat-SRBD.